An Anthracene Carboxamide-Based Fluorescent Probe for Rapid and Sensitive Detection of Mitochondrial Hypochlorite in Living Cells

被引:4
作者
Liu, Xueling [1 ,2 ,3 ]
Wang, Yali [3 ,4 ]
Zhou, Guangshuai [3 ]
Zhang, Wenzhou [1 ,2 ]
机构
[1] Zhengzhou Univ, Affiliated Canc Hosp, Dept Pharm, Zhengzhou 450008, Peoples R China
[2] Henan Canc Hosp, Zhengzhou 450008, Peoples R China
[3] Tianjin Univ, Sch Pharmaceut Sci & Technol, Hlth Sci Platform, Tianjin 300072, Peoples R China
[4] North China Univ Sci & Technol, Coll Pharm, Dept Chem, Tangshan 063000, Peoples R China
来源
BIOSENSORS-BASEL | 2023年 / 13卷 / 09期
关键词
hypochlorite; mitochondria; anthracene carboxyimide; fluorescent probe; HOST-DEFENSE; HOCL; MYELOPEROXIDASE; ACID; INFLAMMATION; MACROPHAGES; LYSOSOME; OXIDANT; ROS;
D O I
10.3390/bios13090883
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Mitochondrial hypochlorite (ClO-) plays important and often contradictory roles in maintaining the redox balance of mitochondria. Abnormal ClO- levels can induce mitochondrial inactivation and further cause cell apoptosis. Herein, we have developed an anthracene carboxyimide-based fluorescent probe mito-ACS for imaging mitochondrial ClO- in living cells. This probe exhibits some distinctive features as excellent resistance to photobleaching, high selectivity and sensitivity, as well as good water solubility. Mito-ACS showed a noticeable fluorescence response toward ClO- with a fast response (within 6 s) and a low detection limit (23 nM). Moreover, the introduction of triphenylphosphonium makes the probe soluble in water and selectively localizes to mitochondria. Furthermore, mito-ACS was successfully applied to image mitochondria ClO- in living cells with low toxicity. Remarkably. the less used fluorophore anthracene carboxyimide exhibiting excellent photostability and desirable optical properties provides a promising application prospect in biological systems.
引用
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页数:11
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