Phytochemical analysis and anticancer activity of the Pithecellobium dulce seed extract in colorectal cancer cells

被引:2
作者
Alhamed, Abdullah S. [1 ]
Alqinyah, Mohammed [1 ]
Alghaith, Adel F. [2 ]
Algahtani, Mohammad M. [1 ]
Alqahtani, Faleh [1 ]
Nasr, Fahd A. [3 ]
Alqahtani, Ali S. [3 ]
Noman, Omar M. [3 ]
Bazaid, Abdulrahman S. [4 ]
AlMalki, Reem Hussain [5 ,6 ]
Rahman, Anas Abdel M. [6 ,7 ]
Alhazzani, Khalid [1 ]
Alanazi, Ahmed Z. [1 ]
机构
[1] King Saud Univ, Coll Pharm, Dept Pharmacol & Toxicol, Riyadh 11451, Saudi Arabia
[2] King Saud Univ, Coll Pharm, Dept Pharmaceut, POB 2457, Riyadh 11451, Saudi Arabia
[3] King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh 11451, Saudi Arabia
[4] Univ Hail, Coll Appl Med Sci, Dept Med Lab Sci, Hail, Saudi Arabia
[5] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia
[6] King Faisal Specialist Hosp & Res Ctr KFSHRC, Ctr Genom Med, Metabol Sect, Dept Clin Genom, Riyadh 11211, Saudi Arabia
[7] Al Faisal Univ, Coll Med, Dept Biochem & Mol Med, Riyadh 11533, Saudi Arabia
关键词
Pithecellobium dulce; colorectal cancer; apoptosis; migration; cytotoxicity; 9-HYDROXYSTEARIC ACID; INHIBITION;
D O I
10.1515/chem-2023-0362
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Colorectal cancer remains a challenging medical issue worldwide, and utilizing natural products and plants to produce novel, effective and safe therapies against this disease is continuously a sought-after strategy. Fruit and leaf extracts of Pithecellobium dulce (P. dulce) showed potential anticancer properties as they induced apoptosis of breast cancer and Dalton's lymphoma ascites cells. Thus, the main objective of the current study is to determine whether the seed extract of P. dulce will affect apoptosis, cell cycle, migration, and inflammation of LoVo colorectal cancer cells. The high-resolution liquid chromatography-mass spectrometry was used to determine the chemical composition of the P. dulce seed extract, which revealed the presence of 35 phytochemicals. The findings of this study indicated a significant cytotoxic effect of seeds of this plant in colorectal cancer characterized by induction of apoptosis, cell cycle arrest, and reduction of migration. In addition, the seed extract suppressed several genes that are essential for cancer progression such as MMP2, MMP9, and IL-8, and, on the other hand, upregulated pro-apoptotic genes such as BAX and P53. This study has established P. dulce as a potential and valuable source for providing future therapies against colorectal cancer and other cancers.
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页数:13
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