Cytocentric measurement for regenerative medicine

被引：1

作者：

Henn, Alicia D. ^{[1
]}

Pereira, Taci ^{[2
]}

Hunsberger, Joshua ^{[3
]}

Mitra, Kunal ^{[4
]}

Izadifar, Zohreh ^{[5
]}

Somara, Sita ^{[6
]}

Lindstrom, Lisa ^{[7
]}

Forest Farb-Horch, Thomas ^{[8
]}

Boy, Jake ^{[9
]}

Muschler, George F. ^{[10
]}

Bauer, Steven R. ^{[11
]}

Yerden, Randy ^{[1
]}

机构：

[1] BioSpherix Ltd, Parish, NY 13131 USA

[2] 3DSystems, Rock Hill, SC USA

[3] RegenMed Dev Org, Winston Salem, NC USA

[4] Florida Inst Technol, Biomed Engn, Melbourne, FL USA

[5] Harvard Univ, Wyss Inst, Boston, MA USA

[6] Vigene Biosci, Rockville, MD USA

[7] Phase Holog Imaging, Lund, Sweden

[8] Thr Biosci Inc, Beverly, MA USA

[9] Sci Bioproc, Pittsburgh, PA USA

[10] Cell X Technol, Cleveland, OH USA

[11] Wake Forest Inst Regenerat Med, Winston Salem, NC USA

来源：

FRONTIERS IN MEDICAL TECHNOLOGY | 2023年 / 5卷

关键词：

regenerative medicine; tissue engineering; cell culture techniques; organoids; stem cells; Sensors; ORGANS-ON-CHIPS; METABOLIC FUNCTION; ALAMAR BLUE; GLUCOSE; LACTATE;

D O I：

10.3389/fmedt.2023.1154653

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Any Regenerative Medicine (RM) business requires reliably predictable cell and tissue products. Regulatory agencies expect control and documentation. However, laboratory tissue production is currently not predictable or well-controlled. Before conditions can be controlled to meet the needs of cells and tissues in culture for RM, we have to know what those needs are and be able to quantify them. Therefore, identification and measurement of critical cell quality attributes at a cellular or pericellular level is essential to generating reproducible cell and tissue products. Here, we identify some of the critical cell and process parameters for cell and tissue products as well as technologies available for sensing them. We also discuss available and needed technologies for monitoring both 2D and 3D cultures to manufacture reliable cell and tissue products for clinical and non-clinical use. As any industry matures, it improves and standardizes the quality of its products. Cytocentric measurement of cell and tissue quality attributes are needed for RM.

引用

页数：6

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