Evaluation of Molecular-level Changes of Programmed Cell Death Ligand-1 after Radiation Therapy in a BALB/c CT26 Colorectal Mouse Tumor Model

被引:1
作者
Chamani, Fateme Khani [1 ]
Shabani, Mahdi [1 ]
Moradi, Afshin [2 ]
Alinejad, Maedeh [1 ]
Jalali, Seyed Amir [1 ,3 ]
机构
[1] Shahid Beheshti Univ Med Sci, Sch Med, Dept Immunol, Tehran, Iran
[2] Shahid Beheshti Univ Med Sci, Sch Med, Dept Pathol, Tehran, Iran
[3] Shahid Beheshti Univ Med Sci, Sch Med, Dept Immunol, Tehran 1985717443, Iran
关键词
Immunohistochemistry; Programmed cell death 1 ligand 1 protein; Radio-frequency therapy; Tumor microenvironments; PD-L1; MICROENVIRONMENT; RADIOTHERAPY; RESISTANCE; OVERCOME; IMMUNITY;
D O I
10.18502/ijaai.v22i2.12677
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
The effects of radiation therapy (RT) for cancer can be systemic and partially mediated by the immune system. However, radiation alone is unlikely to transform an immunosuppressive environment into an immunostimulatory one. Therefore, an effective combination of RT and immunotherapy may provide a new, more efficient treatment approach. Here, we investigated how the expression of programmed cell death-ligand 1 (PD-L1) in the tumor microenvironment varied in different RT regimens with the same biologically effective dose.In this study, female BALB/c mice inoculated with CT26 tumor cells were irradiated with 3 different RT regimens using the same BED of 40 gray (Gy). These included ablative RT (1*15 Gy), hypo-fractionated RT (2*10 Gy), and conventional (Hyper-fractionated) RT (10*3 Gy). PD-L1 expression was analyzed with immunohistochemical staining on days 2 and 20 and when the size of tumors had reached 2 cm2 after RT.All treated groups expressed PD-L1, but the group receiving single ablative high-dose RT showed higher expression compared to the other groups. No significant differences in PD-L1 expression were observed at different times in the same group.These findings showed that different regimens of RT have different effects on the TME, so a combination of RT and immune checkpoint blockade could be clinically used in cancer patients.
引用
收藏
页码:163 / 171
页数:9
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