A novel fluorescence-on fluorescent probe for ONOO- detection in HeLa cells

被引:12
作者
Yan, Hanlei [1 ]
Xu, Xiujuan [1 ]
Li, Jinsa [1 ]
Xie, Peiyao [1 ]
Cao, Wenbo [2 ]
Yang, Xiaopeng [3 ]
Ye, Yong [1 ]
机构
[1] Zhengzhou Univ, Coll Chem, Green Catalysis Ctr, Zhengzhou 450001, Peoples R China
[2] Zhengzhou Univ, Sch Basic Med Sci, Zhengzhou 450001, Peoples R China
[3] Henan Agr Univ, Coll Tobacco Sci, Zhengzhou 450046, Peoples R China
基金
美国国家科学基金会;
关键词
Fluorescent probe; Peroxynitrite; Cell imaging; Ferroptosis; NITRIC-OXIDE; PEROXYNITRITE; FERROPTOSIS; RADICALS;
D O I
10.1016/j.jphotochem.2023.114638
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The reactive nitrogen species peroxynitrite (ONOO-) involve physiological and pathological processes as a significant factor. Develop a probe for monitoring the ONOO- during ferroptosis is very significant. Here, we designed and synthesized a probe TR-ONOO- based on tetraphenylene derivatives and rhodamine analogs for detection of ONOO- with fluorescence-on. Considering the strong oxidative properties of ONOO-, the carbon-carbon double bonds of bridged tetraphenylene derivatives and rhodamine analogs in the TR-ONOO- molecule are oxidized, so that the designed probe can realize fluorescence turn-on. When adding the ONOO- to the probe TR-ONOO- solution, the fluorescence intensity has increased about 40 folds and the color changed from purple to light yellow, enabling naked-eye detection of ONOO-. Furthermore, the probe TR-ONOO- can recognize ONOO- with high selectivity and fast response time. Combining with fluorescence imaging, TR-ONOO- was successfully employed for tracking endogenous ONOO- real-time and the over expression of ONOO- was effectively visualized during erastin-induced ferroptosis.
引用
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页数:6
相关论文
共 43 条
  • [1] Biochemical and cellular toxicology of peroxynitrite: implications in cell death and autoimmune phenomenon
    Ahmad, Rizwan
    Rasheed, Zafar
    Ahsan, Haseeb
    [J]. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, 2009, 31 (03) : 388 - 396
  • [2] Fundamentals on the biochemistry of peroxynitrite and protein tyrosine nitration
    Bartesaghi, Silvina
    Radi, Rafael
    [J]. REDOX BIOLOGY, 2018, 14 : 618 - 625
  • [3] APOPTOSIS AND NECROSIS - 2 DISTINCT EVENTS INDUCED, RESPECTIVELY, BY MILD AND INTENSE INSULTS WITH N-METHYL-D-ASPARTATE OR NITRIC-OXIDE SUPEROXIDE IN CORTICAL CELL-CULTURES
    BONFOCO, E
    KRAINC, D
    ANKARCRONA, M
    NICOTERA, P
    LIPTON, SA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) : 7162 - 7166
  • [4] Coumarin-Based Small-Molecule Fluorescent Chemosensors
    Cao, Duxia
    Liu, Zhiqiang
    Verwilst, Peter
    Koo, Seyoung
    Jangjili, Paramesh
    Kim, Jong Seung
    Lin, Weiying
    [J]. CHEMICAL REVIEWS, 2019, 119 (18) : 10403 - 10519
  • [5] CHAN PH, 1994, BRAIN PATHOL, V4, P59
  • [6] Broadening horizons: the role of ferroptosis in cancer
    Chen, Xin
    Kang, Rui
    Kroemer, Guido
    Tang, Daolin
    [J]. NATURE REVIEWS CLINICAL ONCOLOGY, 2021, 18 (05) : 280 - 296
  • [7] Peroxynitrite scavenging activity of herb extracts
    Choi, HR
    Choi, JS
    Han, YN
    Bae, SJ
    Chung, HY
    [J]. PHYTOTHERAPY RESEARCH, 2002, 16 (04) : 364 - 367
  • [8] A novel reaction-based fluorescence probe for rapid imaging of HClO in live cells, animals, and injured liver tissues
    Deng, Yingzhen
    Feng, Shumin
    Xia, Qingfeng
    Gong, Shengyi
    Feng, Guoqiang
    [J]. TALANTA, 2020, 215
  • [9] Ferroptosis: bug or feature?
    Dixon, Scott J.
    [J]. IMMUNOLOGICAL REVIEWS, 2017, 277 (01) : 150 - 157
  • [10] Ferroptosis: An Iron-Dependent Form of Nonapoptotic Cell Death
    Dixon, Scott J.
    Lemberg, Kathryn M.
    Lamprecht, Michael R.
    Skouta, Rachid
    Zaitsev, Eleina M.
    Gleason, Caroline E.
    Patel, Darpan N.
    Bauer, Andras J.
    Cantley, Alexandra M.
    Yang, Wan Seok
    Morrison, Barclay, III
    Stockwell, Brent R.
    [J]. CELL, 2012, 149 (05) : 1060 - 1072