In vivo longitudinal 920 nm two-photon intravital kidney imaging of a dynamic 2,8-DHA crystal formation and tubular deterioration in the adenine-induced chronic kidney disease mouse model

被引：5

作者：

Choi, Jieun ^{[1
,2
]}

Choi, Min -sun ^{[3
,4
]}

Jeon, Jehwi ^{[1
,2
]}

Moon, Jieun ^{[2
,5
]}

Lee, J. I. N. G. U. ^{[2
,5
]}

Kong, E. U. N. J., I ^{[1
,2
]}

Lucia, Stephani Edwina ^{[1
,2
]}

Hong, S. U. J. U. N. G. ^{[2
,5
]}

Lee, Ji-hye ^{[6
]}

Lee, Eun Young ^{[3
,4
,7
]}

Kim, Pilhan ^{[1
,2
,5
]}

机构：

[1] Korea Adv Inst Sci & Technol KAIST, Grad Sch Med Sci & Engn, Daejeon 34141, South Korea

[2] Korea Adv Inst Sci & Technol KAIST, KI Hlth Sci & Technol KIHST, Daejeon 34141, South Korea

[3] Soonchunhyang Univ, Cheonan Hosp, Dept Internal Med, Cheonan 31151, South Korea

[4] Soonchunhyang Univ, Coll Med, Four Project BK21, Cheonan 31151, South Korea

[5] Korea Adv Inst Sci & Technol KAIST, Grad Sch Nanosci & Technol, Daejeon 34141, South Korea

[6] Soonchunhyang Univ, Cheonan Hosp, Dept Pathol, Cheonan 31151, South Korea

[7] Soonchunhyang Univ, Inst Tissue Regenerat, Coll Med, Cheonan 31151, South Korea

来源：

BIOMEDICAL OPTICS EXPRESS | 2023年 / 14卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

DEFICIENCY; MICROSCOPY;

D O I：

10.1364/BOE.485187

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chronic kidney disease (CKD) is one of the most common renal diseases manifested by gradual loss of kidney function with no symptoms in the early stage. The underlying mechanism in the pathogenesis of CKD with various causes such as high blood pressure, diabetes, high cholesterol, and kidney infection is not well understood. In vivo longitudinal repetitive cellular-level observation of the kidney of the CKD animal model can provide novel insights to diagnose and treat the CKD by visualizing the dynamically changing pathophysiology of CKD with its progression over time. In this study, using two-photon intravital microscopy with a single 920 nm fixed-wavelength fs-pulsed laser, we longitudinally and repetitively observed the kidney of an adenine diet-induced CKD mouse model for 30 days. Interestingly, we could successfully visualize the 2,8-dihydroxyadenine (2,8-DHA) crystal formation with a second-harmonics generation (SHG) signal and the morphological deterioration of renal tubules with autofluorescence using a single 920 nm two-photon excitation. The longitudinal in vivo two-photon imaging results of increasing 2,8-DHA crystals and decreasing tubular area ratio visualized by SHG and autofluorescence signal, respectively, were highly correlated with the CKD progression monitored by a blood test showing increased cystatin C and blood urea nitrogen (BUN) levels over time. This result suggests the potential of label-free second-harmonics generation crystal imaging as a novel optical technique for in vivo CKD progression monitoring. (c) 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement

引用

页码：1647 / 1658

页数：12