PRPF19 mRNA Encodes a Small Open Reading Frame That Is Important for Viability of Human Cells

被引：0

作者：

Shepelev, N. M. ^{[1
,2
]}

Kurochkina, A. O. ^{[2
]}

Dontsova, O. A. ^{[1
,2
,3
,4
]}

Rubtsova, M. P. ^{[1
,2
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia

[2] Moscow MV Lomonosov State Univ, Dept Chem, Moscow, Russia

[3] Lomonosov Moscow State Univ, Belozersky Inst Phys Chem Biol, Moscow, Russia

[4] Skolkovo Inst Sci & Technol, Ctr Mol & Cellular Biol, Moscow, Russia

来源：

DOKLADY BIOCHEMISTRY AND BIOPHYSICS | 2024年 / 515卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

ribosome profiling; upstream ORF; uORF; microprotein; micropeptide; PROTEIN;

D O I：

10.1134/S1607672923700722

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-throughput ribosome profiling demonstrates the translation of thousands of small open reading frames located in the 5 ' untranslated regions of messenger RNAs (upstream ORFs). Upstream ORF can both perform a regulatory function by influencing the translation of the downstream main ORF and encode a small functional protein or microprotein. In this work, we showed that the 5 ' untranslated region of the PRPF19 mRNA encodes an upstream ORF that is translated in human cells. Inactivation of this upstream ORF reduces the viability of human cells.

引用

页码：41 / 47

页数：7

共 4 条

[1] PRPF19 mRNA Encodes a Small Open Reading Frame That Is Important for Viability of Human Cells
N. M. Shepelev
A. O. Kurochkina
O. A. Dontsova
M. P. Rubtsova
Doklady Biochemistry and Biophysics, 2024, 515 : 41 - 47
[2] Truncating the i-leader open reading frame enhances release of human adenovirus type 5 in glioma cells
van den Hengel, Sanne K.
de Vrij, Jeroen
Uil, Taco G.
Lamfers, Martine L.
Smitt, Peter A. E. Sillevis
Hoeben, Rob C.
VIROLOGY JOURNAL, 2011, 8
[3] Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells
Kang, Seok-Jin
Park, In-Byung
Chun, Taehoon
RESEARCH IN VETERINARY SCIENCE, 2021, 140 : 79 - 82
[4] Varicella-Zoster Virus Inhibition of the NF-κB Pathway during Infection of Human Dendritic Cells: Role for Open Reading Frame 61 as a Modulator of NF-κB Activity
Sloan, Elizabeth
Henriquez, Rodney
Kinchington, Paul R.
Slobedman, Barry
Abendroth, Allison
JOURNAL OF VIROLOGY, 2012, 86 (02) : 1193 - 1202

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