Distribution and phylogenetic analysis of Broad bean wilt virus-2 infecting pepper crops in Turkey

被引：0

作者：

Balsak, Selin Ceren ^{[1
]}

Arpaci, Bekir Bulent ^{[2
]}

Buzkan, Nihal ^{[1
]}

机构：

[1] Kahramanmaras Sutcu Imam Univ, Agr Fac, Dept Plant Protect, Kahramanmaras, Turkiye

[2] Kilis 7 Aralik, Agr Fac, Dept Hort, Kilis, Turkiye

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2023年 / 126卷

关键词：

Fabavirus; Broad bean wilt virus-2 (BBWV-2); Capsicum annuum L; VIRUS; IDENTIFICATION;

D O I：

10.1016/j.pmpp.2023.102037

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Broad bean wilt virus 2 (BBWV-2) is a new virus that is affecting a variety of economically important crops around the world and can be transmitted by different species of aphids in a non-persistent manner. The prev-alence of BBWV-2 in pepper has grown quickly over the last decade. In this study, leaf samples from 350 total individual plants were collected from 9 locations in four regions in Turkey, and they were tested with RT-PCR. RT-PCR followed by Blast analyses of the 5 ' UTR region and partial sequence of RNA1 revealed that sixty plants out of 350 (17.1%) were positive for BBWV-2. Twenty-five selected BBWV-2 isolates showed 84-99% nucleotide identity to reference isolates previously reported in the GenBank while this was 94-100% to each other.

引用

页数：5

共 33 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] Establishment of an agroinoculation system for broad bean wilt virus 2 [J].

Atsumi, Go ;

Tomita, Reiko ;

Kobayashi, Kappei ;

Sekine, Ken-Taro .

ARCHIVES OF VIROLOGY, 2013, 158 (07) :1549-1554

[3] Biological and molecular characterization of Tomato spotted wilt orthotospovirus isolates from pepper cultivars with the resistance gene Tsw from Turkey [J].

Balsak, Selin Ceren ;

Buzkan, Nihal .

AUSTRALASIAN PLANT PATHOLOGY, 2022, 51 (06) :543-551

[4]

BENNER CP, 1985, PLANT DIS, V69, P999

[5]

Brunt AA., 1996, VIRUSES PLANTS DESCR

[6]

Buzkan N., 2017, 15 C MED PHYT UN COR, P108

[7] High prevalence of poleroviruses in field-grown pepper in Turkey and Tunisia [J].

Buzkan, Nihal ;

Arpaci, Bulent B. ;

Simon, Vincent ;

Fakhfakh, Hatem ;

Moury, Benoit .

ARCHIVES OF VIROLOGY, 2013, 158 (04) :881-885

[8]

Chang M.U., 1987, KOREAN J PLANT PATHO, V3, P223

[9]

Choi H.S., 2001, KOREA J ELECT MICROS, V31, P215

[10] MUSCLE: multiple sequence alignment with high accuracy and high throughput [J].

Edgar, RC .

NUCLEIC ACIDS RESEARCH, 2004, 32 (05) :1792-1797

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