Phylogenomic analysis in Latilactobacillus sakei by using polymorphisms detected by next-generation sequencing

被引:0
作者
Nishiyama, Chisato [1 ]
Sekiguchi, Suguru [2 ]
Sugihara, Yu [3 ]
Nishikawa, Minami [1 ]
Makita, Natsu [1 ]
Segawa, Tenta [1 ]
Terasaki, Momoka [2 ]
Takagi, Hiroki [1 ]
Koyanagi, Takashi [2 ]
机构
[1] Ishikawa Prefectural Univ, Lab Plant Breeding, 1-308 Suematsu, Nonoichi, Ishikawa 9218836, Japan
[2] Ishikawa Prefectural Univ, Lab Food Microbiol, 1-308 Suematsu, Nonoichi, Ishikawa 9218836, Japan
[3] Kyoto Univ, Lab Crop Evolut, Yoshida Honmachi,Sakyo Ku, Kyoto 6068501, Japan
关键词
Latilactobacillus sakei; phylogenomic analysis; next-generation sequencing (NGS); POPULATION; MICROBIOTA;
D O I
10.12938/bmfh.2022-017
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Latilactobacillus sakei is a lactic acid bacterium used to produce a wide range of fermented food products. To understand their characteristics and adaptability to various nutrient sources, we applied strain-specific, nucleotide-concatenated (SSC) sequences to the phylogenetic analysis of 32 L. sakei strains isolated from various locations and products. SSC sequences were developed by concatenating the polymorphisms detected by whole-genome sequencing. This enabled us to use sufficient polymorphisms and avoid the bias caused by selecting partial sequences, such as that in core genome and multi-locus sequence typing. SSC sequence-based analysis revealed that the phylogenetic relations for L. sakei are based on the different nutrition sources rather than geographical distance.
引用
收藏
页码:138 / 142
页数:5
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