A practical protocol for high-spatial-resolution magnetic resonance angiography for cerebral arteries in rats

被引：0

作者：

Nagahama, Hiroshi ^{[1
,2
]}

Sasaki, Masanori ^{[1
,4
]}

Komatsu, Katsuya ^{[3
]}

Sato, Kaori ^{[2
]}

Katagiri, Yoshimi ^{[2
]}

Kamagata, Masaki ^{[2
]}

Kataoka-Sasaki, Yuko ^{[1
]}

Oka, Shinichi ^{[1
]}

Ukai, Ryo ^{[1
]}

Yokoyama, Takahiro ^{[1
]}

Terada, Kojiro ^{[1
]}

Kobayashi, Masato ^{[1
]}

Kocsis, Jeffery D. ^{[4
,5
]}

Honmou, Osamu ^{[1
,4
]}

机构：

[1] Sapporo Med Univ, Res Inst Frontier Med, Dept Neural Regenerat Med, Sch Med, Sapporo 0608556, Japan

[2] Sapporo Med Univ, Educ & Instrumentat Ctr, Div Radioisotope Res, Biomed Res,Sch Med, Sapporo, Hokkaido 0608556, Japan

[3] Sapporo Med Univ, Dept Neurosurg, Sch Med, Sapporo 0608556, Japan

[4] Yale Univ, Dept Neurol, Sch Med, Neurol, POB 208018, New Haven, CT 06510 USA

[5] Yale Univ, Dept Neurosci, Neurol, Sch Med, POB 208018, New Haven, CT 06510 USA

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2023年 / 386卷

基金：

日本学术振兴会;

关键词：

Magnetic resonance angiography; High-spatial-resolution; Cerebral artery; MR-ANGIOGRAPHY; CONTRAST AGENT; FLOW VELOCITY; SIGNAL LOSS; IN-VIVO; TIME; BRAIN; ECHO;

D O I：

10.1016/j.jneumeth.2023.109784

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Magnetic resonance angiography (MRA) is an important tool in rat models of cerebrovascular dis-ease. Although MRA has long been used in rodents, the image quality is typically not as high as that observed in clinical practice. Moreover, studies on MRA image quality in rats are limited. This study aimed to develop a practical high-spatial-resolution MRA protocol for imaging cerebral arteries in rats.New method: We used the "half position method" regarding coil placement and modified the imaging parameters and image reconstruction method. We applied this new imaging method to measure maturation-related signal changes on rat MRAs.Results: The new practical high-spatial-resolution MRA imaging protocol obtained a signal intensity up to 3.5 times that obtained using a basic coil system, simply by modifying the coil placement method. This method allowed the detection of a gradual decrease in the signal in cerebral vessels with maturation. Comparison with existing methods: A high-spatial-resolution MRA for rats was obtained with an imaging time of approximately 100 min. Comparable resolution and image quality were obtained using the new protocol with an imaging time of 30 minConclusions: The new practical high-spatial-resolution MRA protocol can be implemented simply and successfully to achieve high image quality with an imaging time of approximately 30 min. This protocol will benefit re-searchers performing MRA imaging in cerebral artery studies in rats.

引用

页数：8

共 30 条

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