Experimental approaches in studying active biomolecules modulating fruit ripening: Melatonin as a case study

被引:14
作者
Arabia, Alba [1 ,2 ]
Munoz, Paula [1 ,2 ]
Pallares, Nuria [1 ]
Munne-Bosch, Sergi [1 ,2 ]
机构
[1] Univ Barcelona, Dept Evolutionary Biol Ecol & Environm Sci, Barcelona 08028, Spain
[2] Univ Barcelona, Res Inst Nutr & Food Safety, Barcelona 08028, Spain
关键词
CONFERS CHILLING TOLERANCE; EXOGENOUS MELATONIN; ABSCISIC-ACID; INDOLEACETIC-ACID; TOMATO FRUITS; ROOT-GROWTH; METABOLISM; QUALITY; STRESS; ACCUMULATION;
D O I
10.1093/plphys/kiad106
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Proof from various complementary experimental approaches is needed to assign a specific regulatory function to active biomolecules. Phytohormones are naturally occurring small organic molecules found at low concentrations in plants. They perform essential functions in growth and developmental processes, from organ initiation to senescence, including fruit ripening. These regulatory molecules are studied using different experimental approaches, such as performing exogenous applications, evaluating endogenous levels, and/or obtaining genetically modified lines. Here, we discuss the advantages and limitations of current experimental approaches used to study active biomolecules modulating fruit ripening, focusing on melatonin. Although melatonin has been implicated in fruit ripening in several model fruit crops, current knowledge is affected by the different experimental approaches used, which have given different and sometimes even contradictory results. The methods of application and the doses used have produced different results in studies based on exogenous applications, while different measurement methods and ways of expressing results explain most of the variability in studies using correlative analyses. Furthermore, studies on genetically modified crops have focused on tomato (Solanum lycopersicum L.) plants only. However, TILLING and CRISPR methodologies are becoming essential tools to complement the results from the experimental approaches described above. This will not only help the scientific community better understand the role of melatonin in modulating fruit ripening, but it will also help develop technological advances to improve fruit yield and quality in major crops. The combination of various experimental approaches will undoubtedly lead to a complete understanding of the function of melatonin in fruit ripening in the near future, so that this knowledge can be effectively transferred to the field.
引用
收藏
页码:1747 / 1767
页数:21
相关论文
共 132 条
[11]   Reduced auxin signalling through the cyclophilin gene DIAGEOTROPICA impacts tomato fruit development and metabolism during ripening [J].
Batista-Silva, Willian ;
Carvalho de Oliveira, Alice ;
Oliveira Martins, Auxiliadora ;
Siqueira, Joao Antonio ;
Rodrigues-Salvador, Acacio ;
Omena-Garcia, Rebeca P. ;
Medeiros, David Barbosa ;
Peres, Lazaro Eustaquio Pereira ;
Ribeiro, Dimas Mendes ;
Zsogon, Agustin ;
Fernie, Alisdair R. ;
Nunes-Nesi, Adriano ;
Araujo, Wagner L. .
JOURNAL OF EXPERIMENTAL BOTANY, 2022, 73 (12) :4113-4128
[12]   RNA silencing in plants [J].
Baulcombe, D .
NATURE, 2004, 431 (7006) :356-363
[13]   Melatonin Pre-harvest Treatments Leads to Maintenance of Sweet Cherry Quality During Storage by Increasing Antioxidant Systems [J].
Carrion-Antoli, Alberto ;
Martinez-Romero, Domingo ;
Guillen, Fabian ;
Zapata, Pedro J. ;
Serrano, Maria ;
Valero, Daniel .
FRONTIERS IN PLANT SCIENCE, 2022, 13
[14]   Biotechnological uses of RNAi in plants: risk assessment considerations [J].
Casacuberta, Josep M. ;
Devos, Yann ;
du Jardin, Patrick ;
Ramon, Matthew ;
Vaucheret, Herve ;
Nogue, Fabien .
TRENDS IN BIOTECHNOLOGY, 2015, 33 (03) :145-147
[15]   A sensitive, robust method for determining natural and synthetic hormones in surface and wastewaters by continuous solid-phase extraction-gas chromatography-mass spectrometry [J].
Chafi, Safae ;
Ballesteros, Evaristo .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2022, 29 (35) :53619-53632
[16]   Exogenously applied melatonin stimulates root growth and raises endogenous indoleacetic acid in roots of etiolated seedlings of Brassica juncea [J].
Chen, Qian ;
Qi, Wen-bo ;
Reiter, Russel J. ;
Wei, Wei ;
Wang, Bao-min .
JOURNAL OF PLANT PHYSIOLOGY, 2009, 166 (03) :324-328
[17]   Effects of melatonin treatment on ethanol fermenation and ERF expression in kiwifruit cv. Bruno during postharvest [J].
Cheng, Jiao ;
Zheng, Anran ;
Li, Huihong ;
Huan, Chen ;
Jiang, Tianjia ;
Shen, Shuling ;
Zheng, Xiaolin .
SCIENTIA HORTICULTURAE, 2022, 293
[18]   Nitro-oxidative metabolism during fruit ripening [J].
Corpas, Francisco J. ;
Freschi, Luciano ;
Rodriguez-Ruiz, Marta ;
Mioto, Paulo T. ;
Gonzalez-Gordo, Salvador ;
Palma, Jose M. .
JOURNAL OF EXPERIMENTAL BOTANY, 2018, 69 (14) :3449-3463
[19]  
Davies P.J., 2004, Plant hormones : biosythesis, signal transduction, action!
[20]   Exogenous Melatonin Improves Fruit Quality Features, Health Promoting Antioxidant Compounds and Yield Traits in Tomato Fruits under Acid Rain Stress [J].
Debnath, Biswojit ;
Hussain, Mubasher ;
Li, Min ;
Lu, Xiaocao ;
Sun, Yueting ;
Qiu, Dongliang .
MOLECULES, 2018, 23 (08)