A label-free dual immunosensor for the simultaneous electrochemical determination of CA125 and HE4 biomarkers for the early diagnosis of ovarian cancer

被引：23

作者：

Bilgi Kamac, Melike ^{[1
]}

Altun, Muhammed ^{[1
]}

Yilmaz, Merve ^{[1
]}

Sezginturk, Mustafa Kemal ^{[2
]}

机构：

[1] Cankiri Karatekin Univ, Fac Sci, Chem Dept, TR-18100 Cankiri, Turkiye

[2] Canakkale Onsekiz Mart Univ, Fac Engn, Bioengn Dept, Canakkale, Turkiye

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2023年 / 415卷 / 09期

关键词：

CA125; HE4; Simultaneous determination; Dual electrochemical immunosensors; Ovarian cancer; ANTIGEN; 125; BIOSENSOR; DISEASE; PROTEIN; POINT; PREDICTION; MULTIPLE; MARKER; SIGNAL; RISK;

D O I：

10.1007/s00216-023-04569-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The blood levels of cancer antigen 125 (CA125) and human epididymal secretory protein 4 (HE4) are measured in the diagnosis and progression monitoring of ovarian cancer (OC), and the Risk of Ovarian Malignancy Algorithm (ROMA) score% values are calculated for cancer risk assessment. For the first time, disposable dual screen-printed carbon electrodes modified with reduced graphene oxide, polythionine, and gold nanoparticles were used to fabricate label-free electrochemical dual CA125-HE4 immunosensors for the sensitive, fast, and practical simultaneous determination of CA125 and HE4. DPV and SWV methods were used to simultaneously determine antigens in two different linear ranges (1-100 pg mL(-1) and 1-50 ng mL(-1)). High sensitivity, low LOD, and LOQ were obtained for two linear ranges with a correlation coefficient above 0.99. The application stability of the dual CA125-HE4 immunosensors was determined as 60 days, and the storage stability was determined as 16 weeks. The dual immunosensors exhibited high selectivity in eight different antigen mixtures. The reusability of the dual immunosensors has been tested up to 9 cycles. ROMA score% values for pre-menopausal and post-menopausal status were calculated using the concentration of CA125 and HE4 in the blood serum and assessing OC risk. The disposable dual immunosensors can be used in point-of-care tests for rapid and practical simultaneous determination of CA125 and HE4 with high selectivity, sensitivity, and repeatability.

引用

页码：1709 / 1718

页数：10

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