Hydrogel Loaded with Peptide-Containing Nanocomplexes: Symphonic Cooperation of Photothermal Antimicrobial Nanoparticles and Prohealing Peptides for the Treatment of Infected Wounds

被引：38

作者：

Jia, Qiuye ^{[1
]}

Fu, Zhe ^{[1
]}

Li, Yuansheng ^{[1
]}

Kang, Zijian ^{[1
]}

Wu, Yutong ^{[1
]}

Ru, Zeqiong ^{[1
]}

Peng, Ying ^{[1
]}

Huang, Yubin ^{[1
]}

Luo, Yonglu ^{[1
]}

Li, Wanghongyu ^{[1
]}

Hu, Yiran ^{[1
]}

Sun, Xiaohan ^{[1
]}

Wang, Junyuan ^{[1
]}

Deng, Ziwei ^{[2
]}

Wu, Chunyun ^{[1
]}

Wang, Ying ^{[3
,4
,5
]}

Yang, Xinwang ^{[1
]}

机构：

[1] Kunming Med Univ, Sch Basic Med Sci, Dept Anat & Histol Embryol, Kunming 650500, Yunnan, Peoples R China

[2] Shaanxi Normal Univ, Sch Mat Sci & Engn, Key Lab Appl Surface & Colloid Chem, Shaanxi Key Lab Adv Energy Devices,Natl Minist Edu, Xian 710119, Shaanxi, Peoples R China

[3] Yunnan Minzu Univ, Sch Ethn Med, Key Lab Chem Ethn Med Resources, Kunming 650504, Yunnan, Peoples R China

[4] Yunnan Minzu Univ, Sch Ethn Med, Key Lab Nat Prod Synthet Biol Ethn Med Endophytes, State Ethn Affairs Commiss, Kunming 650504, Yunnan, Peoples R China

[5] Yunnan Minzu Univ, Sch Ethn Med, Minist Educ, Kunming 650504, Yunnan, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2024年 / 16卷 / 11期

基金：

中国国家自然科学基金;

关键词：

CyRL-QN15; melanin nanoparticles; photothermal hydrogel; antibacterial; infectedwound healing; MELANIN NANOPARTICLES; CHITOSAN; SKIN; FABRICATION;

D O I：

10.1021/acsami.3c16061

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Current treatment for chronic infectious wounds is limited due to severe drug resistance in certain bacteria. Therefore, the development of new composite hydrogels with nonantibiotic antibacterial and pro-wound repair is important. Here, we present a photothermal antibacterial composite hydrogel fabricated with a coating of Fe2+ cross-linked carboxymethyl chitosan (FeCMCS) following the incorporation of melanin nanoparticles (MNPs) and the CyRL-QN15 peptide. Various physical and photothermal properties of the hydrogel were characterized. Cell proliferation, migration, cycle, and free-radical scavenging activity were assessed, and the antimicrobial properties of the hydrogel were probed by photothermal therapy. The effects of the hydrogel were validated in a model of methicillin-resistant Staphylococcus aureus (MRSA) infection with full-thickness injury. This effect was further confirmed by changes in cytokines associated with inflammation, re-epithelialization, and angiogenesis on the seventh day after wound formation. The MNPs demonstrated robust photothermal conversion capabilities. The composite hydrogel (MNPs/CyRL-QN15/FeCMCS) promoted keratinocyte and fibroblast proliferation and migration while exhibiting high antibacterial efficacy, effectively killing more than 95% of Gram-positive and Gram-negative bacteria. In vivo study using an MRSA-infected full-thickness injury model demonstrated good therapeutic efficacy of the hydrogel in promoting regeneration and remodeling of chronically infected wounds by alleviating inflammatory response and accelerating re-epithelialization and collagen deposition. The MNPs/CyRL-QN15/FeCMCS hydrogel showed excellent antibacterial and prohealing effects on infected wounds, indicating potential as a promising candidate for wound healing promotion.

引用

页码：13422 / 13438

页数：17

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