Ultrasensitive sensing urinary cystatin C via an interface-engineered graphene extended-gate field-effect transistor for non-invasive diagnosis of chronic kidney disease

被引：23

作者：

Chen, Xiaofen ^{[1
]}

Liang, Yirou ^{[1
]}

Tang, Ning ^{[2
]}

Li, Canye ^{[3
]}

Zhang, Yongheng ^{[1
]}

Xu, Feng ^{[3
]}

Shi, Guoyue ^{[1
]}

Zhang, Min ^{[1
]}

机构：

[1] East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Urban Ecol Proc & Ecorestorat, Dongchuan Rd 500, Shanghai 200241, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Precis Res Ctr Refractory Dis, Sch Med, Shanghai 200025, Peoples R China

[3] Shanghai Jiao Tong Univ, Peoples Hosp 6, Dept Pharm, South Campus, Shanghai 201499, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2024年 / 249卷

基金：

中国国家自然科学基金;

关键词：

Extended -gate field-effect transistor; Urinary cystatin C; Chronic kidney disease; Non-invasive diagnosis; SERUM CREATININE; IMMUNOASSAY;

D O I：

10.1016/j.bios.2024.116016

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Early chronic kidney disease (CKD) has strong concealment and lacks an efficient, non-invasive, and lable-free detection platform. Cystatin C (Cys C) in urine is closely related to the progress of CKD (especially at the early stage), which is an ideal endogenous marker to evaluate the impairment of renal function. Thus, the accurate detection of urinary Cys C (u-Cys C) is great significant for early prevention and treatment and delaying the course of the disease of CKD patients. Herein, we developed an extended-gate field-effect transistor (EG-FET) sensor for ultrasensitive detection of u-Cys C, which consists of a monolithic interface-engineered graphene EG electrode array and a commercially available MOSFET. Laser-induced graphene (LIG) loaded with sputtered Au NPs in the presence of adhesive Cr (Au NPs/Cr/LIG) boosts the electrical performance of the EG electrode. Meanwhile, Au NPs also serve as linkers to immobilize papain that can selectively form protein complexes with Cys C. Supported by the synergistic effect of multilevel interface-engineered graphene, our sensor exhibits a good linear correlation within the u-Cys C concentration range of 5 ag/mu L to 50 ng/mu L with low detection limit of 0.05 ag/mu L. Our work makes accurate, specific and rapid detection of u-Cys C feasible and promising for early screening for CKD.

引用

页数：8

共 43 条

[31] Multi-spot, label-free immunoassay on reflectionless glass [J].