Cfp1 Controls Cardiomyocyte Maturation by Modifying Histone H3K4me3 of Structural, Metabolic, and Contractile Related Genes

被引：2

作者：

Li, Changzhu ^{[1
]}

Zhang, Yang ^{[1
]}

Shen, Jingling ^{[2
]}

Bao, Hairong ^{[1
]}

Zhao, Yue ^{[1
]}

Li, Desheng ^{[1
]}

Li, Sijia ^{[1
]}

Liu, Yining ^{[1
]}

Yang, Jiming ^{[1
]}

Zhou, Zhiwen ^{[1
]}

Gao, Kangyi ^{[1
]}

Zhao, Lexin ^{[1
]}

Pei, Yao ^{[1
]}

Lu, Yanjie ^{[1
]}

Pan, Zhenwei ^{[1
,3
,4
]}

Cai, Benzhi ^{[1
]}

机构：

[1] Harbin Med Univ, Coll Pharm, Dept Pharmacol, Minist Educ,Key Lab Cardiovasc Res,State Key Lab F, Harbin 150086, Heilongjiang, Peoples R China

[2] Chinese Acad Med Sci, Res Unit Noninfect Chron Dis Frigid Zone, 2019 Res Unit 070, Harbin 150086, Heilongjiang, Peoples R China

[3] Wenzhou Univ, Inst Life Sci, Coll Life & Environm Sci, Wenzhou 325035, Peoples R China

[4] Harbin Med Univ, Affiliated Hosp 1, Key Lab Cell Transplantat, Harbin, Peoples R China

来源：

ADVANCED SCIENCE | 2024年 / 11卷 / 11期

基金：

中国国家自然科学基金;

关键词：

cardiomyocyte maturation; Cfp1; hiPSC-CMs; EXPRESSION; COMPLEX;

D O I：

10.1002/advs.202305992

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cardiomyocyte maturation is the final stage of heart development, and abnormal cardiomyocyte maturation will lead to serious heart diseases. CXXC zinc finger protein 1 (Cfp1), a key epigenetic factor in multi-lineage cell development, remains underexplored in its influence on cardiomyocyte maturation. This study investigates the role and mechanisms of Cfp1 in this context. Cardiomyocyte-specific Cfp1 knockout (Cfp1-cKO) mice died within 4 weeks of birth. Cardiomyocytes derived from Cfp1-cKO mice showed an inhibited maturation phenotype, characterized by structural, metabolic, contractile, and cell cycle abnormalities. In contrast, cardiomyocyte-specific Cfp1 transgenic (Cfp1-TG) mice and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) overexpressing Cfp1 displayed a more mature phenotype. Mechanistically, deficiency of Cfp1 led to a reduction in trimethylation on lysine 4 of histone H3 (H3K4me3) modification, accompanied by the formation of ectopic H3K4me3. Furthermore, Cfp1 deletion decreased the level of H3K4me3 modification in adult genes and increased the level of H3K4me3 modification in fetal genes. Collectively, Cfp1 modulates the expression of genes crucial to cardiomyocyte maturation by regulating histone H3K4me3 modification, thereby intricately influencing the maturation process. This study implicates Cfp1 as an important molecule regulating cardiomyocyte maturation, with its dysfunction strongly linked to cardiac disease. Cardiomyocyte maturation is a core process in heart development. The study highlights the critical role of CXXC zinc finger protein 1 (Cfp1) in cardiomyocyte maturation and links its dysfunction to cardiac disease. The findings reveal that Cfp1 promotes cardiomyocyte maturation by regulating trimethylation on lysine 4 of histone H3 (H3K4me3) modification of adult genes, providing potential insights into the treatment of cardiac diseases.image

引用

页数：16

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