Demonstration of seed inoculation of dimer infectious clones of mungbean yellow mosaic India virus-soybean isolate

被引：0

作者：

Gupta, Taruna ^{[1
]}

Singhal, Tripti ^{[1
]}

Dhir, Sunny ^{[2
]}

Chandel, Vanita ^{[1
]}

Rishi, Narayan ^{[1
]}

Raj, S. K. ^{[3
]}

Srivastava, Ashish ^{[1
,4
]}

机构：

[1] Amity Univ Uttar Pradesh, Amity Inst Virol & Immunol, Noida 201313, India

[2] Maharishi Markandeshwar, Dept Biosci & Technol, Cent Res Cell, Ambala 133207, India

[3] B1-66,Sect G, Lucknow 226021, India

[4] Univ Arkansas Syst, Dept Entomol & Plant Pathol, Div Agr, Fayetteville, AR 72701 USA

来源：

3 BIOTECH | 2023年 / 13卷 / 11期

关键词：

Agroinfectious clones; Bipartite begomovirus; Seed inoculation; Detection primers;

D O I：

10.1007/s13205-023-03778-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, the full-length components of mungbean yellow mosaic India virus (MYMIV) DNA-A (MW590720 & MW600934) and DNA-B (MW659819 & MW659820) from a soybean isolate were cloned and sequenced. Nucleotide sequence analysis of both MYMIV components revealed > 96% identity and close ancestry with MYMIV isolates from legumes in Southeast Asia. Furthermore, dimeric infectious clones of MYMIV were generated in the pCAMBIA1302 vector, and a seed infiltration protocol was established for mungbean, soybean, and Nicotiana tabacum. Agroinfiltration induced yellow mosaic symptoms in mungbean and N. tabacum plants 3 weeks post-infiltration, which were further confirmed by PCR using MYMIV-specific DNA primers.

引用

页数：5