Ginsenosides and Ginseng Phenolic Acids Relieve Ionizing Radiation-Induced Pyroptosis and Apoptosis in Intestinal Epithelial Cells

被引:1
作者
Shi, Kai [1 ,2 ,3 ]
Chen, Fei [1 ,2 ,3 ]
Li, Xiang [1 ,2 ,3 ]
Liang, Chen [1 ,2 ,3 ]
Sun, Haiming [4 ]
Yang, Tao [1 ,2 ,3 ]
Zheng, Junnian [2 ,3 ]
Ma, Bo [1 ,2 ,3 ,5 ]
Wei, Liang [1 ,2 ,3 ,5 ]
机构
[1] Xuzhou Med Univ, Canc Inst, 209Tongshan Rd, Xuzhou 221004, Jiangsu, Peoples R China
[2] Xuzhou Med Univ, Affiliated Hosp, Ctr Clin Oncol, Xuzhou, Jiangsu, Peoples R China
[3] Xuzhou Med Univ, Jiangsu Ctr Collaborat & Innovat Canc Biotherapy, Xuzhou, Jiangsu, Peoples R China
[4] Beihua Univ, Tradit Chinese Med Biotechnol Innovat Ctr Jilin Pr, Sch Pharm, Jilin, Peoples R China
[5] Xuzhou Med Univ, Canc Inst, 209 Tongshan Rd, Xuzhou 221004, Jiangsu, Peoples R China
关键词
steroids; phenolics; ginsenosides; ginseng phenolic acids; radiation; pyroptosis; apoptosis; CANCER-CELLS; EXTRACT; RD; ANTIOXIDANT; PATHWAYS; DAMAGE; BCL-2; MEYER; MICE;
D O I
10.1177/1934578X231189954
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Exposure to ionizing radiation (IR) during procedures such as abdominal and pelvic radiotherapy results in severe intestinal injury. In the current study, we demonstrated that a lethal dose of IR triggered intestinal epithelial cell apoptosis and pyroptosis via Caspase-3 cleavage. The ginseng active ingredients, ginsenosides (GS) and ginseng phenolic acids (GPA), significantly inhibited IR-induced apoptosis in IEC-6 cells. GS and GPA also reduced Caspase-3 and GSDME cleavage and HMGB1 and LDH release, thus inhibiting pyroptosis after IR. Moreover, GS- or GPA-treated IEC-6 cells showed reduced reactive oxygen species and DNA damage accumulation. These results suggest that IR induces apoptosis and GSDME-dependent pyroptosis through Caspase-3 activation, which can be relieved by GS and GPA, thus supporting the potential use of GS and GPA in the prevention and treatment of IR-induced intestinal injury.
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页数:14
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