A mismatch amplification mutation assay for specific detection of ciprofloxacin-resistant Neisseria meningitidis

被引：1

作者：

Ota, Yusuke ^{[1
]}

Okada, Reina ^{[1
]}

Takahashi, Hideyuki ^{[2
]}

Saito, Ryoichi ^{[1
,3
]}

机构：

[1] Tokyo Med & Dent Univ, Grad Sch Med & Dent Sci, Dept Mol Microbiol, Tokyo, Japan

[2] Natl Inst Infect Dis, Dept Bacteriol 1, Tokyo, Japan

[3] 1-5-45, Yushima, Bunkyo ku, Tokyo 1138510, Japan

来源：

JOURNAL OF INFECTION AND CHEMOTHERAPY | 2023年 / 29卷 / 05期

基金：

日本学术振兴会;

关键词：

gyrA; Ciprofloxacin-resistant Neisseria meningitidis; Mismatch amplification mutation assay; High single nucleotide discrimination DNA; polymerase;

D O I：

10.1016/j.jiac.2023.02.004

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Meningococcal chemoprophylaxis for people in close contact with patients with invasive meningococcal disease (IMD) is necessary for preventing the spread of Neisseria meningitidis. Ciprofloxacin (CIP) is commonly used to treat IMD. However, CIP-resistant N. meningitidis isolates have rapidly evolved worldwide; therefore, rapid and accurate detection of CIP-resistant N. meningitidis is essential. We developed a mismatch amplification mutation assay for identifying gyrA substitutions T91I and D95Y, associated with reduced CIP susceptibility, using two primer sets to detect these variants. Comparison with gyrA sequencing data showed complete congruency. This method enables reliable detection of CIP-resistant N. meningitidis, thus leading to efficient management and control of IMD infections.

引用

页码：562 / 564

页数：3

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