Exploration of molecularly imprinted polymer (MIP) nanohybrid films as DNA sensors for the detection of porcine

被引:3
作者
Nawaz, Noman [1 ]
Abu Bakar, Nor Kartini [1 ]
Basirun, Wan Jeffrey [1 ,2 ]
Shalauddin, Md [2 ]
Karman, Salmah Binti [3 ]
Ibrahim, Suriani Binti [4 ]
Mahmud, Habibun Nabi Muhammad Ekramul [1 ]
机构
[1] Univ Malaya, Fac Sci, Dept Chem, Kuala Lumpur 50603, Malaysia
[2] Univ Malaya, Nanotechnol & Catalysis Res Ctr NanoCat, Kuala Lumpur 50603, Malaysia
[3] Univ Malaya, Fac Engn, Dept Biomed Engn, Kuala Lumpur 50603, Malaysia
[4] Univ Malaya, Fac Engn, Dept Mech Engn, Kuala Lumpur 50603, Malaysia
关键词
Molecularly imprinted polymers (MIP); Porcine dsDNA; Pyrrole; Titanium dioxide; Nanocomposite film; Biosensor; PORK ADULTERATION; SUS-SCROFA; MODIFIED ELECTRODE; GRAPHENE OXIDE; POLYPYRROLE; NANOPARTICLES; BIOSENSOR; SURFACE; MEAT; PCR;
D O I
10.1007/s11696-022-02530-4
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In the present study, novel molecularly imprinted polymer (MIP) nanocomposite films were prepared for the detection of porcine double-stranded deoxyribonucleic acid (dsDNA) in food samples. A fragment of dsDNA from the pork mitochondrial genome was chosen as a template molecule for porcine identification. The molecularly imprinted polymer (MIP) nanocomposite films of polypyrrole-TiO2 (PPy/TiO2) were synthesized electrochemically on the surface of indium-tin-oxide (ITO) glass electrode. A strong voltammetric (DPV) response was observed for capturing dsDNA from the samples containing porcine. The batch rebinding experiments revealed that PPy/TiO2 MIP possessed excellent binding capacity (Q = 87.44%) and a high imprint factor (2.30) than non-imprinted polymers (NIPs). The selectivity experiments indicated that MIP had excellent recognition selectivity for porcine dsDNA. MIP exhibited higher relative selectivity coefficient (K ' = 4.26 mu M/mu L) toward porcine dsDNA as compared to structural analogue beef dsDNA (K ' = 0.23 mu M/mu L) and chicken dsDNA (K ' = 0.21 mu M/mu L). This could be attributed to the introduction of the imprinted cavities exactly matching the porcine dsDNA on the surface of MIP nanocomposite film. Furthermore, PPy/TiO2 MIP showed a high reusability and low LOD (2.06 mu M) and LOQ (6.89 mu M) values.
引用
收藏
页码:987 / 1003
页数:17
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