First Occurrence of Coffee Leaf Rust Caused by Hemileia vastatrix on Coffee in Saudi Arabia

被引:4
作者
Alhudaib, Khalid [1 ,2 ]
Ismail, Ahmed Mahmoud [1 ,2 ,3 ]
机构
[1] King Faisal Univ, Coll Agr & Food Sci, Dept Arid Land Agr, Al Hasa 31982, Saudi Arabia
[2] King Faisal Univ, Coll Agr & Food Sci, Pests & Plant Dis Unit, Al Hasa 31982, Saudi Arabia
[3] Agr Res Ctr ARC, Plant Pathol Res Inst, Vegetable Dis Res Dept, Giza 12619, Egypt
关键词
Arabian coffee (Coffea arabica L.); Jazan (Saudi Arabia); molecular analysis; ITS; PCR; pathogenicity; coffee rust (H. vastatrix); uredospores; TELIOSPORES; PATHOGEN; IMPACT;
D O I
10.3390/microbiolres15010011
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Coffee leaf rust (CLR) is a major disease of Arabica coffee caused by the biotrophic fungus Hemileia vastatrix. Jazan region in Saudi Arabia has long been one of the last coffee-producing regions in the world free of this disease. In August 2023, CLR was initially observed in coffee plantations located in Fyfa district one of the main coffee-producing mountains of Jazan region. The source of the infection is unknown, however CLR could have entered Jazan from the nearest coffee-producing locations that haven the pathogen. During a scheduled survey in August 2023, symptoms were observed including yellowish-orange lesions that frequently combined to form chlorotic lesions and exhibited the powdery appearance of yellow uredospores on the abaxial surface of leaves. The uredospores and teliospores were microscopically examined and their morphology matched the previously published description for H. vastatrix. The identity of H. vastatrix specimens was further confirmed based on PCR amplification and sequencing of ITS, sharing a 99-100% identity with previously published sequences, as belonging to H. vastatrix. The pathogenicity of H. vastatrix specimens was investigated on Coffee arabica plants under growth chamber conditions, and all were pathogenic relative to control, with 100% of disease incidence, therefore fulfilling Koch's postulates. Based on our findings, this is the first documentation of H. vastatrix causing CLR in Saudi Arabia.
引用
收藏
页码:164 / 173
页数:10
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