Synthetic Cells Revisited: Artificial Cells Construction Using Polymeric Building Blocks

The exponential growth of research on artificial cells and organelles underscores their potential as tools to advance the understanding of fundamental biological processes. The bottom-up construction from a variety of building blocks at the micro- and nanoscale, in combination with biomolecules is key to developing artificial cells. In this review, artificial cells are focused upon based on compartments where polymers are the main constituent of the assembly. Polymers are of particular interest due to their incredible chemical variety and the advantage of tuning the properties and functionality of their assemblies. First, the architectures of micro- and nanoscale polymer assemblies are introduced and then their usage as building blocks is elaborated upon. Different membrane-bound and membrane-less compartments and supramolecular structures and how they combine into advanced synthetic cells are presented. Then, the functional aspects are explored, addressing how artificial organelles in giant compartments mimic cellular processes. Finally, how artificial cells communicate with their surrounding and each other such as to adapt to an ever-changing environment and achieve collective behavior as a steppingstone toward artificial tissues, is taken a look at. Engineering artificial cells with highly controllable and programmable features open new avenues for the development of sophisticated multifunctional systems. The surge in artificial cell and organelle research underscores their potential to advance their understanding of fundamental biology. This review centers on crafting artificial cells from micro- and nanoscale polymer-based compartments, harnessing their varied chemistry for customizable assembly. The authors delve into supramolecular architectures, membrane types, supramolecular structures, functional attributes, communication, and collective behavior, illuminating pathways for building artificial tissues.image