A novel Nitrospira lineage isolated from activated sludge using elevated temperatures

被引:6
作者
Keuter, Sabine [1 ]
Koch, Hanna [2 ]
Nowka, Boris [1 ]
Lipski, Andre [3 ]
Kruse, Myriam [3 ,4 ]
Lucker, Sebastian [2 ]
Spieck, Eva [1 ]
机构
[1] Univ Hamburg, Dept Microbiol & Biotechnol, Ohnhorststr 18, D-22609 Hamburg, Germany
[2] Radboud Univ Nijmegen, Microbiol cluster, RIBES, Heyendaalseweg 135, NL-6525 AJ Nijmegen, Netherlands
[3] Univ Bonn, Dept Food Microbiol & Hyg, Friedrich Hirzebruch Allee7, D-53115 Bonn, Germany
[4] IWW Nord GmbH, Diepholz, Germany
关键词
nitrite oxidation; Nitrospira; nitrification; warm wastewater; niche differentiation; cultivation; NITRITE-OXIDIZING BACTERIUM; COMPLETE NITRIFICATION; POPULATION-STRUCTURE; IN-SITU; SP-NOV; CULTIVATION; COMMUNITY; IDENTIFICATION; OXIDOREDUCTASE; NITROBACTER;
D O I
10.1093/femsle/fnad035
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genus Nitrospira represents the dominant nitrite-oxidizing clade in most wastewater treatment plants (WWTPs) globally, and several Nitrospira strains have been isolated from activated sludge. Using a pre-enrichment strategy with alternating nitrifying and denitrifying conditions, followed by incubation at elevated temperatures, we isolated a novel Nitrospira species, named Nitrospira tepida. This moderately thermophilic species with optimal growth between 37 and 45 degrees C is only distantly related to other Nitrospira and forms a novel lineage VII within the genus, together with few environmental 16S rRNA gene sequences predominantly detected in thermal wastewater or oxygen-limited systems. Genomic and physiological analyses revealed remarkable differences between N. tepida and two other isolates previously obtained from the same WWTP, suggesting niche differentiation between these nitrite oxidizers. N. tepida grows in aggregates, and tolerates nitrite and nitrate concentrations of up to 20 mM and 40 mM, respectively. The K-m value for nitrite of N. tepida is 77 +/- 26 mu M. In summary, this novel Nitrospira lineage seems to be well-adapted for wastewater treatment processes at elevated temperatures and limited aeration, conditions that potentially reduce operational costs of such systems. Insights into physiology, genomic features, and distribution of a moderately thermophilic nitrite oxidizer of the genus Nitrospira from waste water treatment systems.
引用
收藏
页数:10
相关论文
共 69 条
[1]   Temperature influences the population structure of nitrite-oxidizing bacteria in activated sludge [J].
Alawi, M. ;
Off, S. ;
Kaya, M. ;
Spieck, E. .
ENVIRONMENTAL MICROBIOLOGY REPORTS, 2009, 1 (03) :184-190
[2]   Cultivation of a novel cold-adapted nitrite oxidizing betaproteobacterium from the Siberian Arctic [J].
Alawi, Mashal ;
Lipski, Andre ;
Sanders, Tina ;
Eva-Maria-Pfeiffer ;
Spieck, Eva .
ISME JOURNAL, 2007, 1 (03) :256-264
[3]  
[Anonymous], 1977, Lipids, V12, P455
[4]   Freshwater Recirculating Aquaculture System Operations Drive Biofilter Bacterial Community Shifts around a Stable Nitrifying Consortium of Ammonia-Oxidizing Archaea and Comammox Nitrospira [J].
Bartelme, Ryan P. ;
McLellan, Sandra L. ;
Newton, Ryan J. .
FRONTIERS IN MICROBIOLOGY, 2017, 8
[5]   Metabolic versatility of the nitrite-oxidizing bacterium Nitrospira marina and its proteomic response to oxygen-limited conditions [J].
Bayer, Barbara ;
Saito, Mak A. ;
McIlvin, Matthew R. ;
Lucker, Sebastian ;
Moran, Dawn M. ;
Lankiewicz, Thomas S. ;
Dupont, Christopher L. ;
Santoro, Alyson E. .
ISME JOURNAL, 2021, 15 (04) :1025-1039
[6]   A NEW FACULTATIVELY NITRITE OXIDIZING BACTERIUM, NITROBACTER-VULGARIS SP-NOV [J].
BOCK, E ;
KOOPS, HP ;
MOLLER, UC ;
RUDERT, M .
ARCHIVES OF MICROBIOLOGY, 1990, 153 (02) :105-110
[7]   A robust nitrifying community in a bioreactor at 50°C opens up the path for thermophilic nitrogen removal [J].
Courtens, Emilie N. P. ;
Spieck, Eva ;
Vilchez-Vargas, Ramiro ;
Bode, Samuel ;
Boeckx, Pascal ;
Schouten, Stefan ;
Jauregui, Ruy ;
Pieper, Dietmar H. ;
Vlaeminck, Siegfried E. ;
Boon, Nico .
ISME JOURNAL, 2016, 10 (09) :2293-2303
[8]   CRISPRCasFinder, an update of CRISRFinder, includes a portable version, enhanced performance and integrates search for Cas proteins [J].
Couvin, David ;
Bernheim, Aude ;
Toffano-Nioche, Claire ;
Touchon, Marie ;
Michalik, Juraj ;
Neron, Bertrand ;
Rocha, Eduardo P. C. ;
Vergnaud, Gilles ;
Gautheret, Daniel ;
Pourcel, Christine .
NUCLEIC ACIDS RESEARCH, 2018, 46 (W1) :W246-W251
[9]   Exploring the upper pH limits of nitrite oxidation: diversity, ecophysiology, and adaptive traits of haloalkalitolerantNitrospira [J].
Daebeler, Anne ;
Kitzinger, Katharina ;
Koch, Hanna ;
Herbold, Craig W. ;
Steinfeder, Michaela ;
Schwarz, Jasmin ;
Zechmeister, Thomas ;
Karst, Soren M. ;
Albertsen, Mads ;
Nielsen, Per H. ;
Wagner, Michael ;
Daims, Holger .
ISME JOURNAL, 2020, 14 (12) :2967-2979
[10]   Identifying the key taxonomic categories that characterize microbial community diversity using full-scale classification: a case study of microbial communities in the sediments of Hangzhou Bay [J].
Dai, Tianjiao ;
Zhang, Yan ;
Tang, Yushi ;
Bai, Yaohui ;
Tao, Yile ;
Huang, Bei ;
Wen, Donghui .
FEMS MICROBIOLOGY ECOLOGY, 2016, 92 (10)