Green Synthesis of Silver Nanoparticles Using the Cell-Free Supernatant of Haematococcus pluvialis Culture

被引:4
|
作者
Savvidou, Maria G. [1 ,2 ]
Kontari, Evgenia [1 ]
Kalantzi, Styliani [1 ]
Mamma, Diomi [1 ]
机构
[1] Natl Tech Univ Athens, Sch Chem Engn, Biotechnol Lab, Zografou Campus,9 Iroon Polytech Str, Athens 15780, Greece
[2] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA
关键词
green synthesis; microalgae Haematococcus pluvialis; cell-free supernatant; silver nanoparticles; synthesis conditions; antibacterial activity; DUNALIELLA-SALINA; BIOSYNTHESIS; ANTIBACTERIAL; MICROALGAE; EXTRACT;
D O I
10.3390/ma17010187
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The green synthesis of silver nanoparticles (AgNPs) using the cell-free supernatant of a Haematococcus pluvialis culture (CFS) was implemented in the current study, under illumination conditions. The reduction of Ag+ to AgNPs by the CFS could be described by a pseudo-first-order kinetic equation at the temperature range tested. A high reaction rate during synthesis and stable AgNPs were obtained at 45 degrees C, while an alkaline pH (pH = 11.0) and a AgNO3 aqueous solution to CFS ratio of 90:10 (v/v) proved to be the most effective conditions in AgNPs synthesis. A metal precursor (AgNO3) at the concentration range tested (1-5 mM) was the limited reactant in the synthesis process. The synthesis of AgNPs was accomplished under static and agitated conditions. Continuous stirring enhanced the rate of reaction but induced aggregation at prolonged incubation times. Zeta potential and polydispersity index measurements indicated stable AgNPs and the majority of AgNPs formation occurred in the monodisperse phase. The X-ray diffraction (XRD) pattern revealed the face-centered cubic structure of the formed AgNPs, while TEM analysis revealed that the AgNPs were of a quasi-spherical shape with a size from 30 to 50 nm. The long-term stability of the AgNPs could be achieved in darkness and at 4 degrees C. In addition, the synthesized nanoparticles showed antibacterial activity against Escherichia coli.
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页数:23
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