Ultrasensitive quantitation of FLT3-ITD mutation in patients with acute myeloid leukemia using ddPCR

被引：1

作者：

Kojabad, Amir Asri ^{[1
]}

Chegeni, Rouzbeh ^{[2
]}

Rostami, Shaharbano ^{[3
]}

Zaker, Farhad ^{[1
]}

Safa, Majid ^{[1
]}

机构：

[1] Iran Univ Med Sci, Fac Allied Med, Dept Hematol & Blood Banking, Tehran, Iran

[2] Northern Illinois Univ, Coll Hlth & Human Sci, Med Lab Sci Program, De Kalb, IL USA

[3] Univ Tehran Med Sci, Hematol Oncol & Stem Cell Transplantat Res Ctr, Tehran, Iran

来源：

MOLECULAR BIOLOGY REPORTS | 2023年 / 50卷 / 07期

关键词：

FLT3-ITD; ddPCR; Fragment analysis; AML; INTERNAL TANDEM DUPLICATION; DROPLET DIGITAL PCR; AML; REARRANGEMENTS; CYTOGENETICS; PROGNOSIS; DIAGNOSIS; ASSAY;

D O I：

10.1007/s11033-023-08534-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

BackgroundFLT3-ITD mutations occur in 45-50% of cytogenetically normal AML patients. Conventional fragment analysis using capillary electrophoresis is routinely used to quantitate FLT3-ITD mutations. Fragment analysis however has limited sensitivity.Methods and resultsHere, FLT3-ITD was quantified in AML patients using an in-house developed ultra-sensitive droplet digital polymerase chain reaction assay (ddPCR). The allelic ratio of FLT3-ITD was also absolutely measured by both Fragment analysis and ddPCR. The sensitivity of ddPCR in quantitation of FLT3-ITD mutation was superior to Fragment analysis.ConclusionThis study demonstrates the feasibility of using the described in-house ddPCR method to quantify the FLT3-ITD mutation and measure FLT3-ITD AR in AML patients.

引用

页码：6097 / 6105

页数：9

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