Comparative proteomics analyses of female Culexpipiens mosquito gut proteins after sugar and blood feeding using Nano LC-MS

Culex pipiens is a major vector transmitting a variety of arthropod-borne pathogens. Cx pipiens were collected then identified morphologically and by mitochondrial cytochrome oxidase I gene for accurate species confirmation. Mosquito's' gut plays an important role in infection susceptibility. A baseline proteomic dataset is needed to give insights into the physiology of blood feeding and to understand functional proteins in mosquito behavior following a blood meal ultimately leading to establish effective control strategies. As the changes in protein expression of Cx. pipiens gut after sugar and blood feeding have not been investigated, a proteomic analysis of gut tissue was carried out using Nano LC/mass spectrometry (LC-MS) and offline matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-MS. There were 56 functional putative proteins in the gut of sugar fed Cx. pipiens females and 37 proteins gut of blood fed Cx. pipiens females with molecular weight ranging from 800.4 to 2969.4 Da and from 818.3 to 3207.6 Da for sugar and blood fed gut females, respectively. 39 and 20 proteins were uniquely identified in sugar fed and blood fed, respectively, while 17 proteins were common by the two biological groups. Data are available via ProteomeXchange with identifier PXD030533.