Identification of hub genes and pathways of ferroptosis in Fusarium keratitis by bioinformatics methods

被引：8

作者：

Teng, Xingbo ^{[1
]}

Xiong, Xuewei ^{[1
]}

Sha, Xiaoyuan ^{[1
]}

Lei, Yahui ^{[1
]}

Diao, Yuyao ^{[1
]}

Liu, Jiayan ^{[1
]}

Tian, Yuan ^{[1
]}

Liu, Lian ^{[1
]}

Zhong, Jingxiang ^{[1
,2
]}

机构：

[1] Jinan Univ, Affiliated Hosp Jinan Univ 1, Dept Ophthalmol, Guangzhou, Peoples R China

[2] Jinan Univ, Affiliated Hosp Jinan Univ 6, Dongguan, Peoples R China

来源：

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2023年 / 13卷

基金：

中国国家自然科学基金;

关键词：

fungal keratitis; Fusarium keratitis; ferroptosis; bioinformatics; immune; FUNGAL KERATITIS; ORAL VORICONAZOLE; CORNEAL TISSUE; EXPRESSION; DIAGNOSIS; NEUTROPHILS; ASPERGILLUS; INHIBITOR;

D O I：

10.3389/fcimb.2023.1103471

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

BackgroundFungal keratitis is a common blinding eye disease, and Fusarium is one of the main species that cause fungal keratitis. As is well known, oxidative stress plays an important role in Fusarium keratitis and it is also a significant initiating factor of ferroptosis. But the relationship between Fusarium keratitis and ferroptosis is currently unclear. This study aimed to speculate and validate potential ferroptosis-related genes in Fusarium keratitis using bioinformatics analysis, which provided ideas for further research on its specific mechanism and new targets for its treatment. MethodsThe microarray expression profiling dataset (GSE58291) came from Gene Expression Omnibus (GEO). The differentially expressed genes (DEGs) were obtained by the limma package of the R software. The DEGs were performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Then, the DEGs were intersected with the genes in the ferroptosis database. The top 5 hub genes were obtained by the protein-protein interaction (PPI) network analysis and the cytoHubba plug-in of Cytoscape software. The hub genes were subjected to GSEA analysis. Then we analyzed the immune infiltration of the samples by CIBERSORT and ssGSEA algorithm. Finally, we validated the mRNA of hub genes by qPCR. ResultsA total of 1,368 DEGs were identified and 26 ferroptosis-related DEGs were obtained. At the same time, ferroptosis-related pathways were enriched by GO and KEGG using DEGs. HMOX1, CYBB, GPX2, ALOX5 and SRC were obtained by the PPI network analysis and the cytoHubba plug-in of Cytoscape software. The iron metabolism and immune response related pathways were enriched using GSEA. They included hematopoietic cell lineage, lysosome and FC gamma R mediated phagocytosis. T cells follicular helper, monocytes, macrophages and mast cells might play an important role in Fusarium keratitis using analysis of immune infiltration. Finally, qPCR confirmed that the expression of HMOX1, CYBB, ALOX5 mRNA in the DON group was significantly elevated, while the expression of GPX2 were significantly decreased. ConclusionsFerroptosis may play an important role in Fusarium keratitis. HMOX1, CYBB, ALOX5 and GPX2 may be key ferroptosis-related genes in the pathogenesis of Fusarium keratitis.

引用

页数：14

共 50 条

[1] Fusarium keratitis and contact lens wear:: facts and speculations [J].

Ahearn, Donald G. ;

Zhang, Shangtong ;

Stulting, R. Doyle ;

Schwam, Brian L. ;

Simmons, Robert B. ;

Ward, Michael A. ;

Pierce, George E. ;

Crow, Sidney A., Jr. .

MEDICAL MYCOLOGY, 2008, 46 (05) :397-410

[2] Emerging roles of ferroptosis in infectious diseases [J].

Bagayoko, Salimata ;

Meunier, Etienne .

FEBS JOURNAL, 2022, 289 (24) :7869-7890

[3] Regulatory Phenomena in the Glutathione Peroxidase Superfamily [J].

Brigelius-Flohe, Regina ;

Flohe, Leopold .

ANTIOXIDANTS & REDOX SIGNALING, 2020, 33 (07) :498-516

[4] Microbial Keratitis in East Africa: Why are the Outcomes so Poor? [J].

Burton, Matthew J. ;

Pithuwa, Jason ;

Okello, Emily ;

Afwamba, Issac ;

Onyango, Jecinta J. ;

Oates, Francesca ;

Chevallier, Caroline ;

Hall, Anthony B. .

OPHTHALMIC EPIDEMIOLOGY, 2011, 18 (04) :158-163

[5] Prospective Study of the Diagnostic Accuracy of the In Vivo Laser Scanning Confocal Microscope for Severe Microbial Keratitis [J].

Chidambaram, Jaya D. ;

Prajna, Namperumalsamy V. ;

Larke, Natasha L. ;

Palepu, Srikanthi ;

Lanjewar, Shruti ;

Shah, Manisha ;

Elakkiya, Shanmugam ;

Lalitha, Prajna ;

Carnt, Nicole ;

Vesaluoma, Minna H. ;

Mason, Melanie ;

Hau, Scott ;

Burton, Matthew J. .

OPHTHALMOLOGY, 2016, 123 (11) :2285-2293

[6] The Diagnosis and Treatment of Fungal Endophthalmitis: An Update [J].

Danielescu, Ciprian ;

Stanca, Horia Tudor ;

Iorga, Raluca-Eugenia ;

Darabus, Diana-Maria ;

Potop, Vasile .

DIAGNOSTICS, 2022, 12 (03)

[7] Ferroptosis: An Iron-Dependent Form of Nonapoptotic Cell Death [J].

Dixon, Scott J. ;

Lemberg, Kathryn M. ;

Lamprecht, Michael R. ;

Skouta, Rachid ;

Zaitsev, Eleina M. ;

Gleason, Caroline E. ;

Patel, Darpan N. ;

Bauer, Andras J. ;

Cantley, Alexandra M. ;

Yang, Wan Seok ;

Morrison, Barclay, III ;

Stockwell, Brent R. .

CELL, 2012, 149 (05) :1060-1072

[8] Resistant Fusarium Keratitis Progressing to Endophthalmitis [J].

Edelstein, Sean L. ;

Akduman, Levent ;

Durham, Benjamin H. ;

Fothergill, Annette W. ;

Hsu, Hugo Y. .

EYE & CONTACT LENS-SCIENCE AND CLINICAL PRACTICE, 2012, 38 (05) :331-335

[9] Implications of macrophage polarization in autoimmunity [J].

Funes, Samanta C. ;

Rios, Mariana ;

Escobar-Vera, Jorge ;

Kalergis, Alexis M. .

IMMUNOLOGY, 2018, 154 (02) :186-195

[10] To eat or not to eat - the metabolic flavor of ferroptosis [J].

Gao, Minghui ;

Jiang, Xuejun .

CURRENT OPINION IN CELL BIOLOGY, 2018, 51 :58-64

← 1 2 3 4 5 →