Integrated analysis of differentially expressed genes and miRNA expression profiles in dilated cardiomyopathy

被引:1
作者
Chen, Yu [1 ]
Cai, Wen-Ke [2 ]
Yu, Jie [2 ]
Shen, Ming [1 ]
Zhou, Jin-Huan [1 ]
Yang, Sheng-Yu [3 ]
Liu, Wei [1 ]
Lu, Si [4 ]
Shi, Yan-Kun [1 ,5 ]
Yang, Li-Xia [1 ]
机构
[1] 920th Hosp Joint Logist Support Force, PLA, Kunming, Peoples R China
[2] 920th Hosp Joint Logist Support Force, Dept Thoracocardiac Surg, PLA, Kunming, Peoples R China
[3] 920th Hosp Joint Logist Support Force, Dept Urol Surg, PLA, Kunming, Peoples R China
[4] Dali Univ, Dept Clin Med Coll, Dali, Peoples R China
[5] 920th Hosp Joint Logist Support Force Peoples Libe, Dept Cardiol, 212 Daguan Rd, Kunming 650032, Yunnan, Peoples R China
关键词
Dilated cardiomyopathy; Genes; microRNAs; mRNA-miRNA network; Bioinformatics; HEART-FAILURE;
D O I
10.1016/j.heliyon.2024.e25569
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Although dilated cardiomyopathy (DCM) is a prevalent form of cardiomyopathy, the molecular mechanisms underlying its pathogenesis and progression remain poorly understood. It is possible to identify and validate DCM-associated genes, pathways, and miRNAs using bioinformatics analysis coupled with clinical validation methods. Methods: Our analysis was performed using 3 mRNA datasets and 1 miRNA database. We employed several approaches, including gene ontology (GO) analysis, KEGG pathway enrichment analysis, protein-protein interaction networks analysis, and analysis of hub genes to identify critical genes and pathways linked to DCM. We constructed a regulatory network for DCM that involves interactions between miRNAs and mRNAs. We also validated the differently expressed miRNAs in clinical samples (87 DCM ,83 Normal) using qRT-PCR. The miRNAs' clinical value was evaluated by receiver operating characteristic curves (ROCs). Results: 78 differentially expressed genes (DEGs) and 170 differentially expressed miRNAs (DEMs) were associated with DCM. The top five GO annotations were collagen-containing extracellular matrix, cell substrate adhesion, negative regulation of cell differentiation, and inflammatory response. The most enriched KEGG pathways were the Neurotrophin signaling pathway, Thyroid hormone signaling pathway, Wnt signaling pathway, and Axon guidance. In the PPI network, we identified 10 hub genes, and in the miRNA-mRNA regulatory network, we identified 8 hub genes and 15 miRNAs. In the clinical we found 13 miRNAs with an AUC value than Conclusion: Our research offers novel insights into the underlying mechanisms of DCM and has implications for identifying potential targets for diagnosis and treatment of this condition.
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页数:10
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