Anti-inflammatory and anti-oxidant activities of mesenchymal stem cells in chemically induced arthritic rats

被引：0

作者：

Zaky, Mohamed Y. ^{[1
]}

Mohamed, Eman E. ^{[1
]}

Mahmoud, Rehab ^{[2
]}

Halfaya, Fatma Mohamed ^{[3
]}

Farghali, Ahmed ^{[4
]}

Abo El-Ela, Fatma I. ^{[5
]}

机构：

[1] Beni Suef Univ, Fac Sci, Zool Dept, Mol Physiol Div, POB 62521, Bani Suwayf 62521, Egypt

[2] Beni Suef Univ, Fac Sci, Dept Chem Dept, Bani Suwayf 62511, Egypt

[3] Beni Suef Univ, Fac Vet Med, Dept Surg Anesthesiol & Radiol, Bani Suwayf 62511, Egypt

[4] Beni Suef Univ, Fac Postgrad Studies Adv Sci, Mat Sci & Nanotechnol Dept, Bani Suwayf 62511, Egypt

[5] Beni Suef Univ, Fac Vet Med, Dept Pharmacol, Bani Suwayf 62511, Egypt

来源：

MOLECULAR BIOLOGY REPORTS | 2023年 / 50卷 / 12期

关键词：

Rheumatoid arthritis; Mesenchymal stem cells; Inflammation; Oxidative stress; Cartilage degradation; RHEUMATOID-ARTHRITIS; STROMAL CELLS; OXIDATIVE STRESS; BONE-MARROW; DYSFUNCTION; RISK;

D O I：

10.1007/s11033-023-08905-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

BackgroundMesenchymal stem cells (MSCs) have been extensively used as cell-based treatments for decades due to their anti-inflammatory, immunomodulatory, and healing abilities. The intent of our study was to determine the efficacy of MSCs in alleviating rheumatoid arthritis (RA) induced by Complete Freund's adjuvant (CFA) and to investigate the anti-inflammatory and antioxidant characteristics of MSCs.Methods and resultsIntrapedally injecting 0.1 ml of CFA directly into the footpad of the right hind paw daily for 2 days was used to induce RA. Arthritic rats received four doses of MSCs (1 x 106 cells/rat/dose) intravenously through the lateral tail vein. Our results showed that arthritic rats treated with MSCs exhibited reduced levels of paw edema. Furthermore, arthritic rats treated with MSCs exhibited a significant decrease in the levels of RF, CRP, IL-1 beta, TNF-alpha, IL-17 and ADAMTS-5, along with a significant increase in the levels of IL-4 and TIMP-3. Additionally, MSCs significantly reduced the expression of TGF-beta. Both the glutathione (GSH) content and antioxidant activity of GST were enhanced by MSCs, while LPO levels were suppressed.ConclusionThese findings provide further evidence that MSCs are valuable in treating RA, possibly due to their anti-inflammatory and anti-oxidative properties. Thus, MSCs have potential as a more effective therapeutic strategy for treating RA.

引用

页码：9951 / 9961

页数：11

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