Identification of Bioactive Compounds in Plant Extracts of Greek Flora and Their Antimicrobial and Antioxidant Activity

This study examined five species of the Lamiaceae family, Origanum vulgare L., Origanum dictamnus L., Origanum majorana L., Mentha spicata L. and Lavandula angustifolia L., and one species of the Hypericaceae family, Hypericum perforatum L., for their potential to enhance health and wellbeing or to prevent diseases. An analysis of secondary metabolites was performed by mass spectrometry and HPLC coupled with diode array detection to determine the phenolic compounds included in the plant extracts. The samples were evaluated for their total phenolic content, antioxidant potential, ability to prevent DNA scission caused by peroxyl radicals and antimicrobial activity against pathogens (MIC). All the samples, except Lavandula angustifolia L., had a high DPPH radical scavenging activity and showed significant antibacterial activity. Origanum majorana L. demonstrated the lowest IC50 value (10.31 & mu;g & BULL;mL(-1)). Extracts of medicinal herbs had a remarkably high phenolic content varying between 428 and 1512.74 & mu;g GAE/mg of dry extract, with Origanum vulgare L. having the largest amount of polyphenols. Moreover, the plant extracts exhibited a high level of resistance against DNA damage, with Origanum majorana L. showing the greatest level of protection with 98.05% inhibition. According to the results of this study, these plants from the Greek flora provide beneficial effects for health as natural antioxidants. A baseline can be established through the analysis of the findings of this survey for future research on the same plant species.