Recent progress in nucleic acid detection with CRISPR

被引：24

作者：

Liu, Frank X. ^{[1
]}

Cui, Johnson Q. ^{[1
]}

Wu, Zhihao ^{[2
]}

Yao, Shuhuai ^{[1
,3
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Mech & Aerosp Engn, Clear Water Bay, Hong Kong, Peoples R China

[2] Hong Kong Univ Sci & Technol, Interdisciplinary Program Off IPO, IIP Adv Mat, Clear Water Bay, Hong Kong, Peoples R China

[3] Hong Kong Univ Sci & Technol, Dept Chem & Biol Engn, Clear Water Bay, Hong Kong, Peoples R China

来源：

LAB ON A CHIP | 2023年 / 23卷 / 06期

关键词：

RECOMBINASE POLYMERASE AMPLIFICATION; IN-VITRO AMPLIFICATION; DROPLET DIGITAL PCR; ISOTHERMAL AMPLIFICATION; EVOLUTIONARY CLASSIFICATION; DETECTION PLATFORM; DETECTION SYSTEM; DNA-POLYMERASE; ASSAY; DIAGNOSTICS;

D O I：

10.1039/d2lc00928e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in CRISPR-based biotechnologies have greatly expanded our capabilities to repurpose CRISPR for the development of molecular diagnostic systems. The key attribute that allows CRISPR to be widely utilized is its programmable and highly specific nature. In this review, we first illustrate the principle of the class 2 CRISPR nucleases for molecular diagnostics which originates from their immunologic defence systems. Next, we present the CRISPR-based schemes in the application of diagnostics with amplification-assisted or amplification-free strategies. By highlighting some of the recent advances we interpret how general bioengineering methodologies can be integrated with CRISPR. Finally, we discuss the challenges and exciting prospects for future CRISPR-based biosensing development. We hope that this review will guide the reader to systematically learn the start-of-the-art development of CRISPR-mediated nucleic acid detection and understand how to apply the CRISPR nucleases with different design concepts to more general applications in diagnostics and beyond.

引用

页码：1467 / 1492

页数：26

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