Three-dimensional morphological and fluorescent imaging of zebrafish with a continuous-rotational light-sheet microscope

被引：0
作者：
Zhang, Dongmin [1 ,2 ]
Yang, Guang [2 ]
Tan, Yao [2 ]
Chen, Chong [1 ]
Zhang, Jie [2 ]
Li, Hui [1 ,2 ]
机构：
[1] Univ Sci & Technol China, Sch Biomed Engn Suzhou, Div Life Sci & Med, Hefei 230041, Peoples R China
[2] Chinese Acad Sci Suzhou, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Jiangsu, Peoples R China
来源：
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES | 2024年 / 17卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Light-sheet microscope; zebrafish blood vessels; morphology; HIGH-RESOLUTION; DEEP TISSUE;
D O I：
10.1142/S1793545823500220
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Light-sheet fluorescence microscopy (LSFM) has been widely used to image the three-dimensional (3D) structures and functions of various millimeter-size bio-specimen such as zebrafish. However, the sample adsorption and scattering cause shading of the light-sheet illumination, preventing the even 3D image of thick samples. Herein, we report a continuous-rotational light-sheet microscope (CR-LSM) that enables simultaneous 3D bright-field and fluorescence imaging. With a high-accuracy rotational stage, CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation angles. Then, 3D morphology and fluorescent structure were reconstructed with a developed algorithm. Using CR-LSM, zebrafish's whole-fish contour and blood vessel structures were obtained simultaneously.
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页数：8
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