Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repair

被引：37

作者：

Huang, Lin ^{[1
]}

Wang, Wantao ^{[1
,2
]}

Xian, Yiwen ^{[1
]}

Liu, Lei ^{[1
]}

Fan, Jinghao ^{[1
]}

Liu, Hongmei ^{[1
]}

Zheng, Zhaomin ^{[2
]}

Wu, Decheng ^{[1
]}

机构：

[1] Southern Univ Sci & Technol, Dept Biomed Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Spine Surg, Guangzhou 510275, Peoples R China

来源：

MATERIALS TODAY BIO | 2023年 / 22卷

基金：

中国国家自然科学基金;

关键词：

Chitosan; PEG; Injectable hydrogel; Tissue engineering; Intervertebral disc repair; ANNULUS FIBROSUS; LUMBAR DISC; TISSUE; DELIVERY; INTEGRATION; PROPERTY; ADHESIVE; THERAPY; RESTORE;

D O I：

10.1016/j.mtbio.2023.100752

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Intervertebral disc (IVD) degeneration occurred with the increasing age or accidents has puzzled peoples in daily life. To seal IVD defect by injectable hydrogels is a promising method for slowing down IVD degeneration. Herein, we reported a rapidly in situ forming injectable chitosan/PEG hydrogel (CSMA-PEGDA-L) through integrating photo-crosslink of methacrylate chitosan (CSMA) with Schiff base reaction between CSMA and aldehyde polyethylene glycol (PEGDA). The CSMA-PEGDA-L possessed a stronger compressive strength than the photo-crosslinked CSMA-L hydrogel and Schiff-base-crosslinked CSMA-PEGDA hydrogel. This chitosan/PEG hydrogel showed low cytotoxicity from incubation experiments of nucleus pulpous cells. When implanted on the punctured IVD of rat's tail, the CSMA-PEGDA-L hydrogel could well retard the progression of IVD degeneration through physical plugging, powerfully proven by radiological and histological evaluations. This work demon-strated the strategy of in situ injectable glue may be a potential solution for prevention of IVD degeneration.

引用

页数：12

共 52 条

[1] Modulation of Local Overactive Inflammation via Injectable Hydrogel Microspheres [J].

Bian, Jiang ;

Cai, Feng ;

Chen, Hao ;

Tang, Zhenzhou ;

Xi, Kun ;

Tang, Jincheng ;

Wu, Liang ;

Xu, Yichang ;

Deng, Lianfu ;

Gu, Yong ;

Cui, Wenguo ;

Chen, Liang .

NANO LETTERS, 2021, 21 (06) :2690-2698

[2] Tissue-engineered intervertebral discs produce new matrix, maintain disc height, and restore biomechanical function to the rodent spine [J].

Bowles, Robby D. ;

Gebhard, Harry H. ;

Haertl, Roger ;

Bonassar, Lawrence J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (32) :13106-13111

[3] Synthesis and Properties of Hemostatic and Bacteria-Responsive in Situ Hydrogels for Emergency Treatment in Critical Situations [J].

Bu, Yazhong ;

Zhang, Licheng ;

Liu, Jianheng ;

Zhang, Lihai ;

Li, Tongtong ;

Shen, Hong ;

Wang, Xing ;

Yang, Fei ;

Tang, Peifu ;

Wu, Decheng .

ACS APPLIED MATERIALS & INTERFACES, 2016, 8 (20) :12674-12683

[4] Hyaluronic Acid Hydrogels for Biomedical Applications [J].

Burdick, Jason A. ;

Prestwich, Glenn D. .

ADVANCED MATERIALS, 2011, 23 (12) :H41-H56

[5]

Carletti E, 2011, METHODS MOL BIOL, V695, P17, DOI 10.1007/978-1-60761-984-0_2

[6] Injectable self-healing hydrogel with siRNA delivery property for sustained STING silencing and enhanced therapy of intervertebral disc degeneration [J].

Chen, Jiaxin ;

Zhu, Haifeng ;

Zhu, Yutao ;

Zhao, Chenchen ;

Wang, Shengyu ;

Zheng, Yixin ;

Xie, Ziang ;

Jin, Yang ;

Song, Honghai ;

Yang, Linjun ;

Zhang, Jin ;

Dai, Jiayong ;

Hu, Zhijun ;

Wang, Huaiyu .

BIOACTIVE MATERIALS, 2022, 9 :29-43

[7] The Effect of a New Anular Repair After Discectomy in Intervertebral Disc Degeneration An Experimental Study Using a Porcine Spine Model [J].

Chiang, Chang-Jung ;

Cheng, Cheng-Kung ;

Sun, Jui-Sheng ;

Liao, Chun-Jen ;

Wang, Yao-Horng ;

Tsuang, Yang-Hwei .

SPINE, 2011, 36 (10) :761-769

[8] The Functional Role of Interface Tissue Engineering in Annulus Fibrosus Repair: Bridging Mechanisms of Hydrogel Integration with Regenerative Outcomes [J].

DiStefano, Tyler J. ;

Shmukler, Jennifer O. ;

Danias, George ;

Iatridis, James C. .

ACS BIOMATERIALS SCIENCE & ENGINEERING, 2020, 6 (12) :6556-6586

[9] Development of a two-part biomaterial adhesive strategy for annulus fibrosus repair and ex vivo evaluation of implant herniation risk [J].

DiStefano, Tyler J. ;

Shmukler, Jennifer O. ;

Danias, George ;

von Treuheim, Theodor Di Pauli ;

Hom, Warren W. ;

Goldberg, David A. ;

Laudier, Damien M. ;

Nasser, Philip R. ;

Hecht, Andrew C. ;

Nicoll, Steven B. ;

Iatridis, James C. .

BIOMATERIALS, 2020, 258

[10] Self-Healing Conductive Injectable Hydrogels with Antibacterial Activity as Cell Delivery Carrier for Cardiac Cell Therapy [J].

Dong, Ruonan ;

Zhao, Xin ;

Guo, Baolin ;

Ma, Peter X. .

ACS APPLIED MATERIALS & INTERFACES, 2016, 8 (27) :17138-17150

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