The m6A reader ECT1 drives mRNA sequestration to dampen salicylic acid-dependent stress responses in Arabidopsis

被引：40

作者：

Lee, Keun Pyo ^{[1
]}

Liu, Kaiwei ^{[1
,2
]}

Kim, Eun Yu ^{[3
]}

Medina-Puche, Laura ^{[1
]}

Dong, Haihong ^{[1
]}

Di, Minghui ^{[1
,2
]}

Singh, Rahul Mohan ^{[1
]}

Li, Mengping ^{[1
]}

Qi, Shan ^{[1
,2
]}

Meng, Zhuoling ^{[1
,2
]}

Cho, Jungnam ^{[3
,4
]}

Zhang, Heng ^{[1
]}

Lozano-Duran, Rosa ^{[1
]}

Kim, Chanhong ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci CEMPS, Shanghai 200032, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci CEMPS, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China

[4] Chinese Acad Sci, CAS JIC Ctr Excellence Plant & Microbial Sci, Shanghai 200032, Peoples R China

来源：

PLANT CELL | 2024年 / 36卷 / 03期

基金：

中国国家自然科学基金;

关键词：

YTH DOMAIN; P-BODIES; REVEALS; GENE; RECOGNITION; GRANULES; PROTEIN; POLYADENYLATION; TRANSLATION; INFECTION;

D O I：

10.1093/plcell/koad300

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-6-methyladenosine (m(6)A) is a common epitranscriptional mRNA modification in eukaryotes. Thirteen putative m(6)A readers, mostly annotated as EVOLUTIONARILY CONSERVED C-TERMINAL REGION (ECT) proteins, have been identified in Arabidopsis (Arabidopsis thaliana), but few have been characterized. Here, we show that the Arabidopsis m(6)A reader ECT1 modulates salicylic acid (SA)-mediated plant stress responses. ECT1 undergoes liquid-liquid phase separation in vitro, and its N-terminal prion-like domain is critical for forming in vivo cytosolic biomolecular condensates in response to SA or bacterial pathogens. Fluorescence-activated particle sorting coupled with quantitative PCR analyses unveiled that ECT1 sequesters SA-induced m(6)A modification-prone mRNAs through its conserved aromatic cage to facilitate their decay in cytosolic condensates, thereby dampening SA-mediated stress responses. Consistent with this finding, ECT1 overexpression promotes bacterial multiplication in plants. Collectively, our findings unequivocally link ECT1-associated cytosolic condensates to SA-dependent plant stress responses, advancing the current understanding of m(6)A readers and the SA signaling network.

引用

页码：746 / 763

页数：18

共 72 条

[71] Redox rhythm reinforces the circadian clock to gate immune response [J].