N6-adenosine methylation of mRNA integrates multilevel auxin response and ground tissue development in Arabidopsis

被引:6
|
作者
Zemlyanskaya, Elena A. [1 ,2 ]
Zemlianski, Viacheslav [3 ]
Pencik, Ales [4 ,5 ]
Kelley, Dior R. [6 ]
Helariutta, Yka [7 ]
Novak, Ondrej [4 ,5 ]
Ruzicka, Kamil [1 ]
机构
[1] Czech Acad Sci, Inst Expt Bot, Lab Hormonal Regulat Plants, Rozvojova 263, CZ-16000 Prague, Czech Republic
[2] Masaryk Univ, Cent European Inst Technol, Dept Funct Genom & Prote, Kamenice 25, CZ-62500 Brno, Czech Republic
[3] Charles Univ Prague, Fac Sci, Vinicna 1594-7, CZ-12800 Prague, Czech Republic
[4] Palacky Univ, Fac Sci, Lab Growth Regulators, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic
[5] Czech Acad Sci, Inst Expt Bot, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic
[6] Iowa State Univ Sci & Technol, Dept Genet Dev & Cell Biol, 3011 Adv Teaching & Res Bldg, Ames, IA 50011 USA
[7] Univ Helsinki, Inst Biotechnol, Fac Biol & Environm Sci, Wood Dev Grp, Helsinki 00014, Finland
来源
DEVELOPMENT | 2023年 / 150卷 / 19期
关键词
m6A; mRNA methylation; Auxin; Endodermis; SAUR; Arabidopsis; APICAL HOOK DEVELOPMENT; TRANSCRIPTION FACTOR; ADENOSINE METHYLASE; GENE-EXPRESSION; ROOT; EFFLUX; M(6)A; PROTEIN; REGULATOR; SCARECROW;
D O I
10.1242/dev.201775
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
N6-methyl adenosine (m6A) is a widespread internal mRNA modification impacting the expression of numerous genes. Here, we characterize auxin-related defects among the pleiotropic phenotypes of hypomorphic Arabidopsis thaliana mutants with impaired m6A status and reveal that they show strong resistance to exogenously applied auxin. By combining major published m6A datasets, we propose that among high-confidence target transcripts emerge those encoding the main components required for auxin signaling, including the TIR1/AFB auxin receptors and ARF transcriptional regulators. We also observe subtle changes in endogenous levels of indole-3-acetic acid metabolites in these hypomorphic lines, which correlate with the methylation status of indole-3-acetic acid amidohydrolase transcripts. In addition, we reveal that reduced m6A levels lead to defects in endodermal patterning in the primary root arising from impaired timing of periclinal cell divisions. These defects can be reverted by inhibition of auxin signaling. Together, our data underline that m6A likely affects auxindependent processes at multiple levels.
引用
收藏
页数:11
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