Monoterpenoids from the root bark of Acanthopanax gracilistylus and their inhibitory effects on neutrophil elastase, 5-lipoxygenase, andcyclooxygenase-2 in vitro

被引：4

作者：

Yang, Hao-Dong ^{[1
]}

Tang, Zhi-Shu ^{[1
,2
]}

Xue, Tao-Tao ^{[1
]}

Xu, Huai-Li ^{[1
]}

Hou, Bao-Long ^{[1
]}

Zhu, Ya-Ya ^{[1
]}

Su, Zeng-Hu ^{[1
]}

Xu, Hong-Bo ^{[1
]}

机构：

[1] Shaanxi Univ Chinese Med, Coconstruct Collaborat Innovat Ctr Chinese Med Res, State Key Lab Res & Dev Characterist Qin Med Resou, Xianyang 712083, Peoples R China

[2] China Acad Chinese Med Sci, Beijing 100700, Peoples R China

来源：

PHYTOCHEMISTRY | 2023年 / 215卷

基金：

中国国家自然科学基金;

关键词：

Acanthopanacis cortex; Araliaceae; Monoterpenoids; Neutrophil elastase; 5-Lipoxygenase; Cyclooxygenase-2; ALPHA-PINENE; BETA-PINENE; TERPENOIDS; PRODUCTS; BIOTRANSFORMATION; TRANSFORMATIONS; IDENTIFICATION; CONSTITUENTS; OZONOLYSIS; PINANE;

D O I：

10.1016/j.phytochem.2023.113851

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Twenty-four monoterpenoids, including three previously undescribed compounds (1-3), were isolated from the root bark of Acanthopanax gracilistylus W. W. Smith (Acanthopanacis Cortex). Their structures were unambiguously established based on spectroscopic analysis (HR-ESIMS, IR, 1D, and 2D NMR), and the absolute configurations of 1-3 were elucidated by comparing their experimental and calculated electronic circular dichroism spectra. In addition, the structure of 8 was confirmed by single-crystal X-ray diffraction. The inhibitory activities of 1-24 against neutrophil elastase, 5-lipoxygenase, and cyclooxygenase-2 (COX-2) were studied in vitro for the first time, and the results showed that compound 24 possessed a significant inhibitory effect on COX-2 with an IC50 value of 1.53 +/- 0.10 mu\M. This research first reported the presence of monoterpenoids in Acanthopanacis Cortex, including one monoterpenoid 2 with an unusual 4/5 bicyclic lactone system, and compounds 4 and 5 have never been reported in nature.

引用

页数：8

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