Protocol Isolation of adoptively transferred CD8+ T cells in mouse tumor tissues for lipid peroxidation detection

被引：3
|
作者：
Xiao, Liuling [1 ]
Yi, Qing [1 ]
机构：
[1] Houston Methodist Res Inst, Ctr Translat Res Hematol Malignancies, Houston Methodist Canc Ctr, Houston, TX 77030 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 01期
关键词：
FERROPTOSIS;
D O I：
10.1016/j.xpro.2022.101945
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The lipid peroxidation level of tumor-infiltrating CD8+ T cells is crucial for its activity and longevity. Here, we describe a protocol for effective and epitopepreserving dissociation of mouse tumors and subsequent leukocyte purification and lipid peroxidation staining of adoptively transferred CD8+ T cells. We use BODIPY 581/591 C11 to monitor the cellular lipid peroxidation level and detect its fluorescent change by flow cytometry, followed by analysis in FlowJo. This protocol is adaptable to intrinsic CD8+ T cells in tumors as well. For complete details on the use and execution of this protocol, please refer to Xiao et al. (2022)1 and Ma et al. (2021).2
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页数：11
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