Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope

被引：1

作者：

Peng, Ziyuan ^{[1
]}

Jiao, Yuling ^{[2
,3
]}

Wang, Ying ^{[1
]}

机构：

[1] Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China

[2] Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

[3] Peking Univ, Acad Adv Interdisciplinary Studies, Peking Tsinghua Ctr Life Sci, Ctr Quantitat Biol, Beijing 100871, Peoples R China

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 02期

关键词：

Developmental biology; Microscopy; Model Organisms; Plant sciences;

D O I：

10.1016/j.xpro.2023.102217

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Live imaging through confocal laser scanning microscopy enables the recording, analysis, and comparison of the dynamics of shapes and gene expression pat-terns of plant shoot apical meristems (SAMs) or primordia. Here, we provide a protocol to describe the preparation process of imaging Arabidopsis SAMs and primordia using a confocal microscope. We describe steps for dissection, visualization of meristems using dyes and fluorescent proteins, and gain 3D morphology of meristems. We then detail analysis of shoot meristems using time-lapse imaging. For complete details on the use and execution of this protocol, please refer to Peng et al. (2022).1

引用

页数：11