Characterization, estimation of virulence and drug resistance of diarrheagenic escherichia coli (DEC) isolated from Tibetan pigs

被引：3

作者：

Cao, Zhipeng ^{[1
]}

Qi, Ming ^{[1
]}

Shang, Peng ^{[1
]}

Zhang, Hui ^{[2
]}

Nawaz, Shah ^{[3
]}

Ghaffar, Abdul ^{[4
]}

Wu, Qingxia ^{[1
]}

Dong, Hailong ^{[1
]}

机构：

[1] Tibet Agr & Anim Husb Univ, Anim Sci Coll, Linzhi 860000, Peoples R China

[2] South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China

[3] Univ Agr Faisalabad, Fac Vet Sci, Dept Anat, Faisalabad 38000, Pakistan

[4] Islamia Univ Bahawalpur, Fac Chem & Biol Sci, Dept Zool, Bahawalpur 63100, Pakistan

来源：

MICROBIAL PATHOGENESIS | 2023年 / 177卷

关键词：

Tibetan pig; Escherichia coli; Virulence; Drug resistance; HIGH-ALTITUDE ADAPTATION; ANTIMICROBIAL RESISTANCE; POSTWEANING DIARRHEA; PREVALENCE; PIGLETS; GENES; SEROGROUPS; DIVERSITY; BACTERIAL; PROVINCE;

D O I：

10.1016/j.micpath.2023.106046

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, we collected feces of Tibetan piglets from Nyingchi area for isolation, culture, identification, virulence gene analysis and drug resistance analysis of Escherichia Coli. The results demonstrated a 41.3% isolation rate of Diarrheagenic Escherichia Coli from Tibetan pigs with the main phylogenetic groups: group A (68.6%) and group B2 (15.7%). Typical E.coli accounted for 76.5%. The highest detection rates of porcine virulence genes were E.coli heat-resistant enterotoxin STb (58.82%) and F107 fimbrial subunit (23.53%). The highest detection rates of virulence genes from Tibetan pigs were fimC (80.39%) and ompA (76.47%). A drug sensitivity test showed that Diarrheagenic Escherichia Coli from Tibetan pigs had high drug resistance rates to mezlocillin, doxycycline and gentamicin. This study comprehensively analyzed the species composition, virulence and drug resistance of Diarrheagenic Escherichia Coli from Tibetan pigs, which provided a clearer and more targeted idea for the prevention and treatment of yellow and white dysentery in Tibetan pigs in the future.

引用

页数：9

共 50 条

[21] Molecular and Phenotypic Characterization of Diarrheagenic Escherichia coli Strains Isolated from Bacteremic Children [J].

Riveros, Maribel ;

Garcia, Wilfredo ;

Garcia, Coralith ;

Durand, David ;

Mercado, Erik ;

Ruiz, Joaquim ;

Ochoa, Theresa J. .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2017, 97 (05) :1329-1336

[22] Molecular classification and drug resistance analysis of Escherichia coli isolated from poultry in China [J].

Gai, Wenyan ;

Wang, Junwei ;

Wang, Juan ;

Cui, Zhigang ;

Qu, Zhina ;

Cui, Jinghua ;

Du, Xiaoli ;

Huang, Xiumei ;

Zhao, Jianmei .

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2015, 8 (01) :836-844

[23] Virulence genes, antimicrobial resistance profile, phylotyping and pathotyping of diarrheagenic Escherichia coli isolated from children in Southwest Mexico [J].

Tapia-Pastrana, Gabriela ;

Rojas-Bautista, Metztli ;

Hernandez-Perez, Pilar ;

Santiago-Martinez, Olegario ;

Gomez-Rodriguez, Lucia C. ;

Terrazas-Luna, Victor M. ;

Montes-Yedra, Jacobo ;

Bautista-Avendano, Alfonso A. ;

Garcia-Lopez, Eduardo S. ;

Leon-Sicairos, Nidia ;

Angulo-Zamudio, Uriel A. ;

Canizalez-Roman, Adrian .

PLOS ONE, 2024, 19 (03)

[24] Characterization of virulence determinants and phylogenetic background of multiple and extensively drug resistant Escherichia coli isolated from different clinical sources in Egypt [J].

El-Baz, Rana ;

Said, Heba Shehta ;

Abdelmegeed, Eman Salama ;

Barwa, Rasha .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2022, 106 (03) :1279-1298

[25] Genomic insights into virulence, antimicrobial resistance, and adaptation acumen of Escherichia coli isolated from an urban environment [J].

Saini, Poorvi ;

Bandsode, Viraj ;

Singh, Anuradha ;

Mendem, Suresh Kumar ;

Semmler, Torsten ;

Alam, Munirul ;

Ahmed, Niyaz .

MBIO, 2024, 15 (03)

[26] Antimicrobial resistance, diarrheagenic and avian pathogenic virulence genes in Escherichia coli from poultry feed and the ingredients [J].

Rossato, J. M. ;

Brito, B. G. ;

Kobayashi, R. K. T. ;

Koga, V. L. ;

Sarmiento, J. J. P. ;

Nakazato, G. ;

Lopes, L. F. D. ;

Balsan, L. A. G. ;

Grassotti, T. T. ;

Brito, K. C. T. .

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA, 2019, 71 (06) :1968-1976

[27] Genomic and Virulence Characterization of Intrauterine Pathogenic Escherichia coli With Multi-Drug Resistance Isolated From Cow Uteri With Metritis [J].

Ma, Zhengxin ;

Ginn, Amber ;

Kang, Minyoung ;

Galvao, Klibs N. ;

Jeong, Kwangcheol Casey .

FRONTIERS IN MICROBIOLOGY, 2018, 9

[28] Antibiotic resistance, virulence factors, and phylogenetic groups of Escherichia coli isolated from hospital wastewater: A case study in the west of Iran [J].

Bozorgomid, Arezoo ;

Lorestani, Roya Chegene ;

Rostamian, Mosayeb ;

Zargaran, Fatemeh Nemati ;

Shahvaisi-Zadeh, Zhila ;

Akya, Alisha .

ENVIRONMENTAL HEALTH ENGINEERING AND MANAGEMENT JOURNAL, 2023, 10 (02) :131-139

[29] Characterization of Uropathogenic Escherichia coli Reveals Hybrid Isolates of Uropathogenic and Diarrheagenic (UPEC/DEC) E. coli [J].

Tanabe, Rodrigo H. S. ;

Dias, Regiane C. B. ;

Orsi, Henrique ;

de Lira, Daiany R. P. ;

Vieira, Melissa A. ;

dos Santos, Luis F. ;

Ferreira, Adriano M. ;

Rall, Vera L. M. ;

Mondelli, Alessandro L. ;

Gomes, Tania A. T. ;

Camargo, Carlos H. ;

Hernandes, Rodrigo T. .

MICROORGANISMS, 2022, 10 (03)

[30] Characterization of Escherichia coli pathogenicity and drug resistance in yolk peritonitis [J].

Li, Qingqing ;

Fang, Weile ;

Chen, Shupeng ;

Li, Guyue ;

Jiang, Chenxi ;

Zhuang, Yu ;

Li, Lin ;

Liu, Pei ;

Guo, Xiaoquan ;

Hu, Guoliang ;

Liu, Ping ;

Gao, Xiaona .

POULTRY SCIENCE, 2024, 103 (07)

← 1 2 3 4 5 →