Effectiveness of BMP-2 and PDGF-BB Adsorption onto a Collagen/Collagen-Magnesium-Hydroxyapatite Scaffold in Weight-Bearing and Non-Weight-Bearing Osteochondral Defect Bone Repair: In Vitro, Ex Vivo and In Vivo Evaluation

被引：14

作者：

Xu, Jietao ^{[1
]}

Fahmy-Garcia, Shorouk ^{[1
,2
]}

Wesdorp, Marinus A. A. ^{[1
]}

Kops, Nicole ^{[1
]}

Forte, Lucia ^{[3
]}

De Luca, Claudio ^{[3
]}

Misciagna, Massimiliano Maraglino ^{[3
]}

Dolcini, Laura ^{[3
]}

Filardo, Giuseppe ^{[4
]}

Labberte, Margot ^{[5
]}

Vancikova, Karin ^{[5
]}

Kok, Joeri ^{[6
]}

van Rietbergen, Bert ^{[6
]}

Nickel, Joachim ^{[7
]}

Farrell, Eric ^{[2
]}

Brama, Pieter A. J. ^{[5
]}

van Osch, Gerjo J. V. M. ^{[1
,8
,9
]}

机构：

[1] Univ Med Ctr, Dept Orthoped & Sports Med, Erasmus MC, NL-3015 GD Rotterdam, Netherlands

[2] Univ Med Ctr, Dept Oral & Maxillofacial Surg, Erasmus MC, NL-3015 GD Rotterdam, Netherlands

[3] Fin Ceram Faenza SpA, I-48018 Faenza, Italy

[4] IRCCS Rizzoli Orthopaed Inst, Appl & Translat Res Ctr, I-40136 Bologna, Italy

[5] Univ Coll Dublin, Sch Vet Med, Dublin D04 V1W8, Ireland

[6] Eindhoven Univ Technol, Dept Biomed Engn, NL-5612 AZ Eindhoven, Netherlands

[7] Univ Hosp Wurzburg, Dept Tissue Engn & Regenerat Med, D-97070 Wurzburg, Germany

[8] Univ Med Ctr, Dept Otorhinolaryngol, Erasmus MC, NL-3015 GD Rotterdam, Netherlands

[9] Delft Univ Technol, Dept Biomech Engn, NL-2628 CD Delft, Netherlands

来源：

JOURNAL OF FUNCTIONAL BIOMATERIALS | 2023年 / 14卷 / 02期

基金：

爱尔兰科学基金会; 欧盟地平线“2020”;

关键词：

tissue engineering; regenerative medicine; osteochondral lesion; biocompatible materials; bone morphogenetic proteins; platelet-derived growth factor; animal model; weight-bearing; OSTEOGENIC GENE-EXPRESSION; COLLAGEN-BASED SCAFFOLDS; MESENCHYMAL STEM-CELLS; MORPHOGENETIC PROTEIN-2; ARTICULAR-CARTILAGE; REGENERATION; TISSUE; DIFFERENTIATION; CHONDROGENESIS; ENVIRONMENT;

D O I：

10.3390/jfb14020111

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Despite promising clinical results in osteochondral defect repair, a recently developed bi-layered collagen/collagen-magnesium-hydroxyapatite scaffold has demonstrated less optimal subchondral bone repair. This study aimed to improve the bone repair potential of this scaffold by adsorbing bone morphogenetic protein 2 (BMP-2) and/or platelet-derived growth factor-BB (PDGF-BB) onto said scaffold. The in vitro release kinetics of BMP-2/PDGF-BB demonstrated that PDGF-BB was burst released from the collagen-only layer, whereas BMP-2 was largely retained in both layers. Cell ingrowth was enhanced by BMP-2/PDFG-BB in a bovine osteochondral defect ex vivo model. In an in vivo semi-orthotopic athymic mouse model, adding BMP-2 or PDGF-BB increased tissue repair after four weeks. After eight weeks, most defects were filled with bone tissue. To further investigate the promising effect of BMP-2, a caprine bilateral stifle osteochondral defect model was used where defects were created in weight-bearing femoral condyle and non-weight-bearing trochlear groove locations. After six months, the adsorption of BMP-2 resulted in significantly less bone repair compared with scaffold-only in the femoral condyle defects and a trend to more bone repair in the trochlear groove. Overall, the adsorption of BMP-2 onto a Col/Col-Mg-HAp scaffold reduced bone formation in weight-bearing osteochondral defects, but not in non-weight-bearing osteochondral defects.

引用

页数：20