The Effect of Poria cocos Polysaccharide PCP-1C on M1 Macrophage Polarization via the Notch Signaling Pathway

被引：15

作者：

Hu, Xuerui ^{[1
]}

Hong, Bangzhen ^{[1
]}

Shan, Xiaoxiao ^{[1
]}

Cheng, Yue ^{[1
]}

Peng, Daiyin ^{[1
,2
,3
]}

Hu, Rongfeng ^{[4
,5
]}

Wang, Lei ^{[1
,2
,4
]}

Chen, Weidong ^{[1
,2
,3
,4
]}

机构：

[1] Anhui Univ Chinese Med, Sch Pharm, Hefei 230001, Peoples R China

[2] Anhui Prov Key Lab Chinese Med Formula, Hefei 230001, Peoples R China

[3] Anhui Univ Chinese Med, Inst Tradit Chinese Med Resource, Hefei 230001, Peoples R China

[4] Anhui Univ Chinese Med, Anhui Prov Key Lab Pharmaceut Preparat Technol & A, Hefei 230001, Peoples R China

[5] Anhui Univ Chinese Med, Key Lab, Xinan Med Minist Educ, Hefei 230001, Peoples R China

来源：

MOLECULES | 2023年 / 28卷 / 05期

基金：

美国国家科学基金会;

关键词：

Poria cocos; homogeneous polysaccharide; macrophage polarization; Notch signaling pathway; KAPPA-B; ACTIVATION; KINASE; SCLEROTIUM; EXPRESSION; GROWTH; CELLS; MACA; IL-6;

D O I：

10.3390/molecules28052140

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The homogeneous galactoglucan PCP-1C extracted from Poria cocos sclerotium has multiple biological activities. The present study demonstrated the effect of PCP-1C on the polarization of RAW 264.7 macrophages and the underlying molecular mechanism. Scanning electron microscopy showed that PCP-1C is a detrital-shaped polysaccharide with fish-scale patterns on the surface, with a high sugar content. The ELISA assay, qRT-PCR assay, and flow cytometry assay showed that the presence of PCP-1C could induce higher expression of M1 markers, including tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and interleukin-12 (IL-12), when compared with the control and the LPS group, and it caused a decrease in the level of interleukin-10 (IL-10), which is the marker for M2 macrophages. At the same time, PCP-1C induces an increase in the CD86 (an M1 marker)/CD206 (an M2 marker) ratio. The results of the Western blot assay showed that PCP-1C induced activation of the Notch signaling pathway in macrophages. Notch1, ligand Jagged1, and Hes1 were all up-regulated with the incubation of PCP-1C. These results indicate that the homogeneous Poria cocos polysaccharide PCP-1C improves M1 macrophage polarization through the Notch signaling pathway.

引用

页数：15

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