An alternative approach to validation of liquid chromatography-mass spectrometry methods for the quantification of endogenous compounds

Despite the progress in the quantification of xenobiotics, the development and validation of methods de-signed for endogenous substances still remain challenging due to the natural presence of the analytes in a biological matrix, leading to the inability to obtain a blank sample. Several generally recognized pro-cedures are described to solve this issue, like using surrogate or analyte-depleted matrices or surrogate analytes. However, the workflows used do not always meet the requirements for developing a reliable analytical method or are cost-intensive.This study aimed to design an alternative approach for preparing validation reference samples us-ing authentic analytical standards while preserving the nature of the biological matrix and solving the problem with the inherent presence of analyzed compounds in a studied matrix. The methodology used is based on the standard-addition type procedure. However, unlike the original method, the addition is modified according to a previously measured basal concentration of monitored substances in the pooled biological sample to obtain a predefined concentration in reference samples according to the European Medicines Agency (EMA) validation guideline. The study shows the advantages of described approach on an example of LC-MS/MS analysis of 15 bile acids in human plasma and compares it with other methods commonly used in this field.The method was successfully validated according to the EMA guideline with lower limit of quan-tification of 5 nmol/L and linearity in the range of 5 -20 0 0 nmol/L. Finally, the method was used in a metabolomic study on a cohort of pregnant women ( n = 28) to confirm intrahepatic cholestasis, the major liver disease observed in pregnancy.& COPY; 2023 Elsevier B.V. All rights reserved.