Integrative analysis of negatively regulated miRNA-mRNA axes for esophageal squamous cell carcinoma

被引:1
作者
Peng, Shuang [1 ]
Zhang, Shiyu [1 ]
Fan, Xingchen [2 ]
Zhu, Jingfeng [3 ]
Liu, Cheng [4 ]
Yue, Yulin [5 ]
Wang, Tongshan [1 ]
Zhu, Wei [1 ]
机构
[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Oncol, Nanjing, Jiangsu, Peoples R China
[2] First Peoples Hosp Lianyungang, Dept Geriatr, Lianyungang, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Affiliated Hosp 1, Dept Nephrol, Nanjing, Jiangsu, Peoples R China
[4] Nanjing Med Univ, Affiliated Hosp 1, Dept Gastroenterol, Nanjing, Jiangsu, Peoples R China
[5] Nanjing Med Univ, Childrens Hosp, Dept Lab, Nanjing, Jiangsu, Peoples R China
关键词
Biomarkers; miRNA-mRNA interaction; esophageal squamous cell carcinoma; TCGA; GEO; PCR; RELATIVE GENE-EXPRESSION; CANCER; SIGNATURE; DATABASE;
D O I
10.3233/CBM-220309
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
BACKGROUND: MicroRNAs regulating mRNA expression by targeting at mRNAs is known constructive in tumor occurrence, immune escape, and metastasis. OBJECTIVE: This research aims at finding negatively regulatory miRNA-mRNA pairs in esophageal squamous cell carcinoma (ESCC). METHODS: GENE expression data of The Cancer Genome Atlas (TCGA) and GEO database were employed in differently expressed RNA and miRNA (DE-miRNAs/DE-mRNAs) screening. Function analysis was conducted with DAVID-mirPath. MiRNA-mRNA axes were identified by MiRTarBase and TarBase and verified in esophageal specimen by real-time reverse transcription polymerase chain reaction (RT-qPCR). Receiver operation characteristic (ROC) curve and Decision Curve Analysis (DCA) were applied in miRNA-mRNA pairs predictive value estimation. Interactions between miRNA-mRNA regulatory pairs and immune features were analyzed using CIBERSORT. RESULTS: Combining TCGA database, 4 miRNA and 10 mRNA GEO datasets, totally 26 DE-miRNAs (13 up and 13 down) and 114 DE-mRNAs (64 up and 50 down) were considered significant. MiRTarBase and TarBase identified 37 reverse regulation miRNA-mRNA pairs, 14 of which had been observed in esophageal tissue or cell line. Through analysis of RT-qPCR outcome, miR-106b-5p/KIAA0232 signature was chosen as characteristic pair of ESCC. ROC and DCA verified the predictive value of model containing miRNA-mRNA axis in ESCC. Via affecting mast cells, miR-106b-5p/KIAA0232 may contribute to tumor microenvironment. CONCLUSIONS: The diagnostic model of miRNA-mRNA pair in ESCC was established. Their complex role in ESCC pathogenesis especially tumor immunity was partly disclosed.
引用
收藏
页码:191 / 203
页数:13
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