Synthesis and Characterization of Chitosan Acetylcholine Nanoparticles for Neural Disorders Associated with Cancer Treatment

Neurological diseases caused by chemotherapy treatments are associated with weak neurotransmission of neurotransmitters. Degradation of neurotransmitters leads to neurological disorders such as peripheral neuropathic pain. Acetylcholine esterase degrades the acetylcholine neurotransmitters in the synapse, leading to neuropathic pain. Drugs inhibiting acetylcholine esterase result in side effects. The use of nanoparticles for safeguarding acetylcholine is one of the better methods. Chitosan and neurotransmitters combination may cure neurological disorders. This research focused on preparing and characterizing chitosan acetylcholine nanoparticles targeting neuropathic pain and in silico analysis of sodium ion channels for neuropathic pain. Zeta sizer analysis, HR-TEM, and FT-IR have characterized nanoparticles of chitosan-acetylcholine. The chitosan ACh nanoparticle has an irregularly spherical shape and a size of fewer than 200 nm by transmission electron microscopy. Chitosan ACh nanoparticles exhibited good antioxidant potential. Invitro effect on human neuroblastoma cells shows reduced cytotoxicity compared to cancer cells. In silico binding on the sodium-gated ions channels show efficient binding, which leads to a reduction in the neuropathic pain.{GRAPHIACAL ABSTRACT}