LncRNA DGUOK-AS1 facilitates non-small cell lung cancer growth and metastasis through increasing TRPM7 stability via m6A modification

被引：17
作者：
Feng, Yimin [1 ]
Wu, Fengjuan [2 ]
Wu, Yuanning [3 ]
Guo, Zihan [3 ]
Ji, Xiang [3 ]
机构：
[1] Shandong Univ, Hosp 2, Dept Clin Lab, 247 Beiyuan St, Jinan 250033, Shandong, Peoples R China
[2] Heze Municipal Hosp, Dept Pulm & Crit Care Med, Heze 274031, Shandong, Peoples R China
[3] Shandong First Med Univ, Affiliated Hosp 1, Shandong Inst Resp Dis, Dept Pulm & Crit Care Med, 16766 Jingshi Rd, Jinan 250014, Shandong, Peoples R China
来源：
TRANSLATIONAL ONCOLOGY | 2023年 / 32卷
关键词：
NSCLC; m6A modification; Growth; Metastasis; DGUOK-AS1;
D O I：
10.1016/j.tranon.2023.101661
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: N6-methyladenosine (m6A) modification plays key roles in tumor progression. LncRNA deoxyguanosine kinase antisense RNA 1 (DGUOK-AS1) has been reported as a promoter in tumors, but its role and mechanism in non-small cell lung cancer (NSCLC) development remain uncertain. Methods: Cell proliferation, migration, invasion and angiogenesis were investigated via CCK-8, colony formation, transwell, and tube formation assays, respectively. The location of DGUOK-AS1 was detected via FISH assay. The interaction relationship among DGUOK-AS1, IGF2BP2 and TRPM7 was confirmed by RIP and MeRIP assays. The effects of DGUOK-AS1 on NSCLC growth and metastasis in vivo were investigated using xenograft and pulmonary metastatic models. Results: DGUOK-AS1 was upregulated in NSCLC. DGUOK-AS1 silencing inhibited NSCLC cell proliferation, migration, invasion and angiogenesis. DGUOK-AS1 was mostly expressed in cytoplasm, and positively regulated IGF2BP2. METTL3/IGF2BP2 axis could increase TRPM7 mRNA stability in m6A-dependent manner. TRPM7 overexpression reversed the inhibitive function of DGUOK-AS1 silencing on NSCLC development. DGUOK-AS1 knockdown suppressed NSCLC cell growth and metastasis in nude mice. Conclusion: DGUOK-AS1 silencing restrains NSCLC cell growth and metastasis through decreasing TRPM7 stability via regulation of the METTL3/IGF2BP2-mediated m6A modification.
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页数：11
相关论文
共 55 条
[41]   DGUOK-AS1 promotes cervical squamous cell carcinoma progression by suppressing miR-499a-5p that targets SPRR1B in vitro [J].
Song, Yan ;
Pan, Hongjuan ;
Yang, Ling ;
Fan, Yan ;
Zhang, Hui ;
Pan, Meichen ;
Zhang, Yali .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021, 585 :177-184
[42]   Molecular Pathology of Primary Non-small Cell Lung Cancer [J].
Suster, David Ilan ;
Mino-Kenudson, Mari .
ARCHIVES OF MEDICAL RESEARCH, 2020, 51 (08) :784-798
[43]   GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses [J].
Tang, Zefang ;
Li, Chenwei ;
Kang, Boxi ;
Gao, Ge ;
Li, Cheng ;
Zhang, Zemin .
NUCLEIC ACIDS RESEARCH, 2017, 45 (W1) :W98-W102
[44]   The m6A RNA methylation regulates oncogenic signaling pathways driving cell malignant transformation and carcinogenesis [J].
Uddin, Mohammad Burhan ;
Wang, Zhishan ;
Yang, Chengfeng .
MOLECULAR CANCER, 2021, 20 (01)
[45]   The potential role of RNA N6-methyladenosine in Cancer progression [J].
Wang, Tianyi ;
Kong, Shan ;
Tao, Mei ;
Ju, Shaoqing .
MOLECULAR CANCER, 2020, 19 (01)
[46]   DGUOK-AS1 promotes cell proliferation in cervical cancer via acting as a ceRNA of miR-653-5p [J].
Wu, Nanchang ;
Song, Honglin ;
Ren, Yaoyao ;
Tao, Shan ;
Li, Shiting .
CELL BIOCHEMISTRY AND FUNCTION, 2020, 38 (07) :870-879
[47]  
Xu L, 2021, AM J CANCER RES, V11, P5282
[48]   Suppression of TRPM7 Inhibited Hypoxia-Induced Migration and Invasion of Androgen-Independent Prostate Cancer Cells by Enhancing RACK1-Mediated Degradation of HIF-1α [J].
Yang, Fei ;
Cai, Jiarong ;
Zhan, Hailun ;
Situ, Jie ;
Li, Wenbiao ;
Mao, Yunhua ;
Luo, Yun .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2020, 2020
[49]   LCAT1 is an oncogenic LncRNA by stabilizing the IGF2BP2-CDC6 axis [J].
Yang, Juze ;
Qian, Xinyi ;
Qiu, Qiongzi ;
Xu, Lingling ;
Pan, Meidie ;
Li, Jia ;
Ren, Jiayi ;
Lu, Bingjian ;
Qiu, Ting ;
Chen, Enguo ;
Ying, Kejing ;
Zhang, Honghe ;
Lu, Yan ;
Liu, Pengyuan .
CELL DEATH & DISEASE, 2022, 13 (10)
[50]   New insights into the interplay between long non-coding RNAs and RNA-binding proteins in cancer [J].
Yao, Zi-Ting ;
Yang, Yan-Ming ;
Sun, Miao-Miao ;
He, Yan ;
Liao, Long ;
Chen, Kui-Sheng ;
Li, Bin .
CANCER COMMUNICATIONS, 2022, 42 (02) :117-140
123456