LncRNA DGUOK-AS1 facilitates non-small cell lung cancer growth and metastasis through increasing TRPM7 stability via m6A modification

被引：16
作者：
Feng, Yimin [1 ]
Wu, Fengjuan [2 ]
Wu, Yuanning [3 ]
Guo, Zihan [3 ]
Ji, Xiang [3 ]
机构：
[1] Shandong Univ, Hosp 2, Dept Clin Lab, 247 Beiyuan St, Jinan 250033, Shandong, Peoples R China
[2] Heze Municipal Hosp, Dept Pulm & Crit Care Med, Heze 274031, Shandong, Peoples R China
[3] Shandong First Med Univ, Affiliated Hosp 1, Shandong Inst Resp Dis, Dept Pulm & Crit Care Med, 16766 Jingshi Rd, Jinan 250014, Shandong, Peoples R China
来源：
TRANSLATIONAL ONCOLOGY | 2023年 / 32卷
关键词：
NSCLC; m6A modification; Growth; Metastasis; DGUOK-AS1;
D O I：
10.1016/j.tranon.2023.101661
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: N6-methyladenosine (m6A) modification plays key roles in tumor progression. LncRNA deoxyguanosine kinase antisense RNA 1 (DGUOK-AS1) has been reported as a promoter in tumors, but its role and mechanism in non-small cell lung cancer (NSCLC) development remain uncertain. Methods: Cell proliferation, migration, invasion and angiogenesis were investigated via CCK-8, colony formation, transwell, and tube formation assays, respectively. The location of DGUOK-AS1 was detected via FISH assay. The interaction relationship among DGUOK-AS1, IGF2BP2 and TRPM7 was confirmed by RIP and MeRIP assays. The effects of DGUOK-AS1 on NSCLC growth and metastasis in vivo were investigated using xenograft and pulmonary metastatic models. Results: DGUOK-AS1 was upregulated in NSCLC. DGUOK-AS1 silencing inhibited NSCLC cell proliferation, migration, invasion and angiogenesis. DGUOK-AS1 was mostly expressed in cytoplasm, and positively regulated IGF2BP2. METTL3/IGF2BP2 axis could increase TRPM7 mRNA stability in m6A-dependent manner. TRPM7 overexpression reversed the inhibitive function of DGUOK-AS1 silencing on NSCLC development. DGUOK-AS1 knockdown suppressed NSCLC cell growth and metastasis in nude mice. Conclusion: DGUOK-AS1 silencing restrains NSCLC cell growth and metastasis through decreasing TRPM7 stability via regulation of the METTL3/IGF2BP2-mediated m6A modification.
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页数：11
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共 55 条
  • [1] An adipose lncRAP2-Igf2bp2 complex enhances adipogenesis and energy expenditure by stabilizing target mRNAs
    Alvarez-Dominguez, Juan R.
    Winther, Sally
    Hansen, Jacob B.
    Lodish, Harvey F.
    Knoll, Marko
    [J]. ISCIENCE, 2022, 25 (01)
  • [2] [Anonymous], 2021, MOL THER NUCL ACIDS, DOI DOI 10.3892/MMR.2020.11711
  • [3] [Anonymous], EXP CELL RES
  • [4] Systemic Therapy for Locally Advanced and Metastatic Non-Small Cell Lung Cancer A Review
    Arbour, Kathryn C.
    Riely, Gregory J.
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2019, 322 (08): : 764 - 774
  • [5] Insulin-like growth factor 2 mRNA-binding proteins (IGF2BPs): post-transcriptional drivers of cancer progression?
    Bell, Jessica L.
    Waechter, Kristin
    Muehleck, Britta
    Pazaitis, Nikolaos
    Koehn, Marcel
    Lederer, Marcell
    Huettelmaier, Stefan
    [J]. CELLULAR AND MOLECULAR LIFE SCIENCES, 2013, 70 (15) : 2657 - 2675
  • [6] Long non-coding RNA ILF3-AS1 facilitates hepatocellular carcinoma progression by stabilizing ILF3 mRNA in an m6A-dependent manner
    Bo, Changwen
    Li, Na
    He, Li
    Zhang, Sujing
    An, Yonghui
    [J]. HUMAN CELL, 2021, 34 (06) : 1843 - 1854
  • [7] Targeting Strategy to Proliferating Cell Nuclear Antigen (PCNA) as an Effective Strategy to Inhibit Tumor Cell Proliferation
    Cardano, Miriana
    Tribioli, Carla
    Prosperi, Ennio
    [J]. CURRENT CANCER DRUG TARGETS, 2020, 20 (04) : 240 - 252
  • [8] HDAC1 overexpression promoted by METTL3-IGF2BP2 inhibits FGF21 expression in metabolic syndrome-related liver injury
    Chen, Yunjiang
    Cai, Kaiyu
    Du, Yueling
    Liu, Zixiong
    Gong, Yanchun
    [J]. BIOCHEMISTRY AND CELL BIOLOGY, 2023, 101 (01) : 52 - 63
  • [9] The Important Role of N6-methyladenosine RNA Modification in Non-Small Cell Lung Cancer
    Cheng, Yue
    Wang, Meiqi
    Zhou, Junliang
    Dong, Huanhuan
    Wang, Shuqing
    Xu, Hui
    [J]. GENES, 2021, 12 (03)
  • [10] IGF2BP2 promotes the progression of colorectal cancer through a YAP-dependent mechanism
    Cui, Jie
    Tian, Jiale
    Wang, Weiwei
    He, Tao
    Li, Xin
    Gu, Chenzheng
    Wang, Lixin
    Wu, Jian
    Shang, Anquan
    [J]. CANCER SCIENCE, 2021, 112 (10) : 4087 - 4099
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