Elucidation of lipid nanoparticle surface structure in mRNA vaccines

被引：23

作者：

Wang, Mingzhang Maple ^{[1
]}

Wappelhorst, Caitlin N. ^{[1
]}

Jensen, Erika L. ^{[1
]}

Chi, Ying-Chih Thomas ^{[1
]}

Rouse, Jason C. ^{[2
]}

Zou, Qin ^{[1
]}

机构：

[1] Pfizer Inc, Analyt Res & Dev, BioTherapeut Pharmaceut Sci, 875 Chesterfield Pkwy West, Chesterfield, MO 63017 USA

[2] Pfizer Inc, Analyt Res & Dev, BioTherapeut Pharmaceut Sci, 1 Burtt Rd, Andover, MA 01810 USA

来源：

SCIENTIFIC REPORTS | 2023年 / 13卷 / 01期

关键词：

POLYETHYLENE-GLYCOL; TRIGGERED RELEASE; NMR-SPECTROSCOPY; EX-VIVO; SIRNA; PH; IMPACT;

D O I：

10.1038/s41598-023-43898-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Lipid nanoparticles (LNPs) have been used as a carrier for messenger RNA (mRNA) vaccines. Surface properties of LNPs are important to the stability and function of mRNA vaccines. Polyethylene-glycol (PEG) is a functional lipid at the surface of LNPs that improves colloidal stability, increases circulation time, and impacts cellular uptake. In this study, we explore in-depth lipid composition at the surface of mRNA-LNPs using high-field nuclear magnetic resonance (NMR) spectroscopy. Our results provide a unique surface lipid profile of intact LNPs identifying PEG chains and partial ionizable lipids are present with quantification capability. The surface PEG density is determined to reveal the brush-like conformation on the surface of mRNA-LNPs. Furthermore, we implement a diffusion NMR strategy for routine testing of formulated drug products during drug development. Comparative NMR analysis of different vaccine preparations and stability samples provides a global view of the mRNA-LNP surface structure for enhanced product knowledge.

引用

页数：8

共 32 条

[1]

[Anonymous], TQ Analyst Software

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