A Chemical Counterpart to the Resolution Step of Nature's Intein-Mediated Protein Splicing

被引：2
|
作者：
Dhayalan, Balamurugan [1 ,2 ]
Kent, Stephen B. H. [1 ]
Fetter-Pruneda, Ingrid [3 ,4 ]
机构：
[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA
[2] Indiana Univ Sch Med, Dept Biochem & Mol Biol, 635 Barnhill Rd, Indianapolis, IN 46202 USA
[3] Rockefeller Univ, Lab Social Evolut & Behav, New York, NY 10065 USA
[4] Univ Nacl Autonoma Mexico, Dept Biol Celular & Fisiol, Inst Invest Biomed, Mexico City 04510, Mexico
来源：
ACS CHEMICAL BIOLOGY | 2023年 / 19卷 / 01期
关键词：
INSULIN; PEPTIDES;
D O I：
10.1021/acschembio.3c00590
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In the course of an attempted total chemical synthesis of the ant insulin-like peptide-2 (ILP2) protein molecule, specific cleavage of a backbone peptide bond in a branched ester-linked polypeptide chain with concomitant peptide splicing was observed. The side reaction was investigated in model compounds. Here, we postulate a chemical mechanism for this novel polypeptide backbone cleavage reaction as a chemical counterpart to the resolution step of biochemical intein-mediated protein splicing.
引用
收藏
页码：9 / 14
页数：6
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