The Effect of Silencing Fatty Acid Elongase 4 and 6 Genes on the Proliferation and Migration of Colorectal Cancer Cells

被引：3

作者：

Czumaj, Aleksandra ^{[1
]}

Kobiela, Jaroslaw ^{[2
,3
]}

Mika, Adriana ^{[1
]}

Pappou, Emmanouil ^{[4
]}

Sledzinski, Tomasz ^{[1
]}

机构：

[1] Med Univ Gdansk, Fac Pharm, Dept Pharmaceut Biochem, PL-80211 Gdansk, Poland

[2] Med Univ Gdansk, Fac Med, Dept Gen Endocrine & Transplant Surg, PL-80211 Gdansk, Poland

[3] Med Univ Gdansk, Fac Med, Dept Surg Oncol, Gdansk, Poland

[4] Mem Sloan Kettering Canc Ctr, Dept Surg, New York, NY 10065 USA

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 24期

关键词：

colorectal cancer; lipid metabolism; fatty acid elongation; siRNA; cell proliferation; cell migration; INHIBITOR; MICRORNAS; DISCOVERY; PATHWAYS; ROLES;

D O I：

10.3390/ijms242417615

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Colorectal cancer (CRC) cells show some alterations in lipid metabolism, including an increased fatty acid elongation. This study was focused on investigating the effect of a small interfering RNA (siRNA)-mediated decrease in fatty acid elongation on CRC cells' survival and migration. In our study, the elongase 4 (ELOVL4) and elongase 6 (ELOVL6) genes were observed to be highly overexpressed in both the CRC tissue obtained from patients and the CRC cells cultured in vitro (HT-29 and WiDr cell lines). The use of the siRNAs for ELOVL4 and ELOVL6 reduced cancer cell proliferation and migration rates. These findings indicate that the altered elongation process decreased the survival of CRC cells, and in the future, fatty acid elongases can be potentially good targets in novel CRC therapy.

引用

页数：10

共 45 条

[1] Epigenetic and genetic features of 24 colon cancer cell lines [J].

Ahmed, D. ;

Eide, P. W. ;

Eilertsen, I. A. ;

Danielsen, S. A. ;

Eknaes, M. ;

Hektoen, M. ;

Lind, G. E. ;

Lothe, R. A. .

ONCOGENESIS, 2013, 2 :e71-e71

[2] Colon Cancer: A Clinician's Perspective in 2019 [J].

Ahmed, Monjur .

GASTROENTEROLOGY RESEARCH, 2020, 13 (01) :1-10

[3] Multi-omics of 34 colorectal cancer cell lines - a resource for biomedical studies [J].

Berg, Kaja C. G. ;

Eide, Peter W. ;

Eilertsen, Ina A. ;

Johannessen, Bjarne ;

Bruun, Jarle ;

Danielsen, Stine A. ;

Bjornslett, Merete ;

Meza-Zepeda, Leonardo A. ;

Eknaes, Mette ;

Lind, Guro E. ;

Myklebost, Ola ;

Skotheim, Rolf I. ;

Sveen, Anita ;

Lothe, Ragnhild A. .

MOLECULAR CANCER, 2017, 16

[4] Cancer Focus Lipid metabolism and cancer [J].

Bian, Xueli ;

Liu, Rui ;

Meng, Ying ;

Xing, Dongming ;

Xu, Daqian ;

Lu, Zhimin .

JOURNAL OF EXPERIMENTAL MEDICINE, 2021, 218 (01)

[5]

Bond LM., 2016, Biochemistry of Lipids, Lipoproteins and Membranes, P185, DOI DOI 10.1016/B978-0-444-63438-2.00006-7

[6] ELOVL5 Is a Critical and Targetable Fatty Acid Elongase in Prostate Cancer [J].

Centenera, Margaret M. ;

Scott, Julia S. ;

Machiels, Jelle ;

Nassar, Zeyad D. ;

Miller, Deanna C. ;

Zinonos, Irene ;

Dehairs, Jonas ;

Burvenich, Ingrid J. G. ;

Zadra, Giorgia ;

Chetta, Paolo M. ;

Bango, Clyde ;

Evergren, Emma ;

Ryan, Natalie K. ;

Gillis, Joanna L. ;

Mah, Chui Yan ;

Tieu, Terence ;

Hanson, Adrienne R. ;

Carelli, Ryan ;

Bloch, Katarzyna ;

Panagopoulos, Vasilios ;

Waelkens, Etienne ;

Derua, Rita ;

Williams, Elizabeth D. ;

Evdokiou, Andreas ;

Cifuentes-Rius, Anna ;

Voelcker, Nicolas H. ;

Mills, Ian G. ;

Tilley, Wayne D. ;

Scott, Andrew M. ;

Loda, Massimo ;

Selth, Luke A. ;

Swinnen, Johannes, V ;

Butler, Lisa M. .

CANCER RESEARCH, 2021, 81 (07) :1704-1718

[7] Small interfering RNA for cancer treatment: overcoming hurdles in delivery [J].

Charbe, Nitin Bharat ;

Amnerkar, Nikhil D. ;

Ramesh, B. ;

Tambuwala, Murtaza M. ;

Bakshi, Hamid A. ;

Aljabali, Alaa A. A. ;

Khadse, Saurabh C. ;

Satheeshkumar, Rajendran ;

Satija, Saurabh ;

Metha, Meenu ;

Chellappan, Dinesh Kumar ;

Shrivastava, Garima ;

Gupta, Gaurav ;

Negi, Poonam ;

Dua, Kamal ;

Zacconi, Flavia C. .

ACTA PHARMACEUTICA SINICA B, 2020, 10 (11) :2075-2109

[8] Interfering with disease: a progress report on siRNA-based therapeutics [J].

de Fougerolles, Antonin ;

Vornlocher, Hans-Peter ;

Maraganore, John ;

Lieberman, Judy .

NATURE REVIEWS DRUG DISCOVERY, 2007, 6 (06) :443-453

[9] Novel Cellular Functions of Very Long Chain-Fatty Acids: Insight From ELOVL4 Mutations [J].

Deak, Ferenc ;

Anderson, Robert E. ;

Fessler, Jennifer L. ;

Sherry, David M. .

FRONTIERS IN CELLULAR NEUROSCIENCE, 2019, 13

[10] G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences [J].

Faul, Franz ;

Erdfelder, Edgar ;

Lang, Albert-Georg ;

Buchner, Axel .

BEHAVIOR RESEARCH METHODS, 2007, 39 (02) :175-191

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