Determination of carbon-14 in marine biota using oxidation combustion and gel suspension liquid scintillation counting

In this study, we report a new analytical method for determination of 14C in marine biota utilizing oxidation combustion separation combined with CaCO3 suspension of precipitates for liquid scintillation counting (LSC). The main factors affecting the oxidation combustion efficiency of tube combustion furnace and the counting efficiency of the LSC were systematically investigated and optimized. Under the optimal combustion conditions, the combustion recovery of carbon ranged from 85.6 % to 92.4 % for five marine samples. And the method achieved a detection limit of 0.13 Bq/g for 14C and repeatability of 3.9-9.1 %. The analytical turnover time was 6 h, and up to six samples could be processed simultaneously. Assessment of uncertainty components showed that the uncertainty in counting was the largest contributor, followed by combustion recovery and counting efficiency. The developed method enables reliable measurement of 14C in marine biota, providing valuable analytical tool for risk assessment purposes.