Rapid and precise genome engineering in a naturally short-lived vertebrate

被引：10

作者：

Bedbrook, Claire N. ^{[1
,2
]}

Nath, Ravi D. ^{[1
]}

Nagvekar, Rahul ^{[1
]}

Deisseroth, Karl ^{[2
,3
,4
]}

Brunet, Anne ^{[1
,5
,6
]}

机构：

[1] Stanford Univ, Dept Genet, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Bioengn, Stanford, CA USA

[3] Stanford Univ, Dept Psychiat & Behav Sci, Stanford, CA USA

[4] Stanford Univ, Howard Hughes Med Inst, Stanford, CA USA

[5] Glenn Labs Biol Aging Stanford, Stanford, CA 94305 USA

[6] Stanford Univ, Wu Tsai Neurosci Inst, Stanford, CA 94305 USA

来源：

ELIFE | 2023年 / 12卷

基金：

美国国家卫生研究院;

关键词：

Nothobranchius furzeri; African killifish; aging; CRISPR; Cas9-mediated knock-in; genetics; genome engineering; Other; AFRICAN TURQUOISE KILLIFISH; EXPRESSION; EVOLUTION; MODEL; ZEBRAFISH; DYNAMICS; INSIGHTS; PROTEIN;

D O I：

10.7554/eLife.80639

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here, we develop a rapid and precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show its efficient application to precisely insert fluorescent reporters of different sizes at various genomic loci in order to drive cell-type- and tissue-specific expression. This knock-in method should allow the establishment of humanized disease models and the development of cell-type-specific molecular probes for studying complex vertebrate biology.

引用

页数：21

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